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PMID: 1731975 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterisation of PHSP1, a cDNA encoding a mitochondrial HSP70 from Pisum sativum.

Plant molecular biology ·Vol. 18 ·No. 1 ·1992-01-00 ·Pages 23-32

Watts FZ, Walters AJ, Moore AL

Abstract

A pea cDNA clone, PHSP1, encoding a member of the HSP70 gene family has been isolated. DNA sequence analysis indicates that the protein encoded by PHSP1 is a homologue of the mitochondrial HSP70 proteins, SSP1 from Schizosaccharomyces pombe and SSC1 from S. cerevisiae. It contains an amino-terminal extension of 50 amino acids, rich in basic and hydroxyl amino acids, similar to other plant mitochondrial leader sequences. Western blot analysis indicates that the PHSP1 protein is associated only with mitochondria and not with any other sub-cellular organelle or cytoplasm. Further confirmation of its location within mitochondria was obtained from in vitro protein translocation experiments into purified Pisum sativum mitochondria. It was observed that the precursor protein was efficiently imported and that it is processed to produce a protein with an Mr of the anticipated size of the mature protein. Results are discussed with respect to the structure and function of the mitochondrial HSP70 protein.

Related Genes
PHSP1
MeSH Terms
Amino Acid Sequence Blotting, Western Fabaceae/genetics,metabolism Heat-Shock Proteins/chemistry,genetics Mitochondria/metabolism Molecular Sequence Data Plants, Medicinal Translocation, Genetic/genetics
Chemicals
Heat-Shock Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Watts F Z
Microbial Genetics Group, School of Biological Sciences, University of Sussex, Brighton, UK.
Walters A J
Moore A L
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00018453
Published
1992-01-00
Pages
23-32
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X54739
External Links
PubMed source DOI: 10.1007/BF00018453 View journal details
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