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PMID: 2151717 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sorting of precursor proteins between isolated spinach leaf mitochondria and chloroplasts.

Plant molecular biology ·Vol. 14 ·No. 6 ·1990-06-00 ·Pages 977-82

Whelan J, Knorpp C, Glaser E

Abstract

The precursors of the F1-ATPase beta-subunits from Nicotiana plumbaginifolia and Neurospora crassa were imported into isolated spinach (Spinacia oleracea L.) leaf mitochondria. Both F1 beta precursors were imported and processed to mature size products. No import of the mitochondrial precursor proteins into isolated intact spinach chloroplasts was seen. Moreover, the precursor of the 33 kDa protein of photosynthetic water-splitting enzyme was not imported into the leaf mitochondria. This study provides the first experimental report of in vitro import of precursor proteins into plant mitochondria isolated from photosynthetic tissue and enables studies of protein sorting between mitochondria and chloroplasts in a system which is homologous with respect to organelles. The results suggest a high organellar specificity in the plant cell for the cytoplasmically synthesized precursor proteins.

MeSH Terms

Biological Transport, Active Chloroplasts/metabolism Fungal Proteins/metabolism Mitochondria/metabolism Neurospora crassa/metabolism Plant Proteins/metabolism Plants/metabolism Plants, Toxic Protein Precursors/metabolism Proton-Translocating ATPases/metabolism Tobacco/metabolism Vegetables/metabolism

Chemicals

Fungal Proteins Plant Proteins Protein Precursors Proton-Translocating ATPases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Whelan J

Department of Biochemistry, Arrhenius Laboratories, University of Stockholm, Sweden.

Knorpp C

Glaser E

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00019394

Published

1990-06-00

Pages

977-82

Language

English

Region

Netherlands

NLM ID

9106343

Subset

External Links

PubMed source DOI: 10.1007/BF00019394 View journal details

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Sorting of precursor proteins between isolated spinach leaf mitochondria and chloroplasts.

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