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PMID: 2265609 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A precursor protein partly translocated into yeast mitochondria is bound to a 70 kd mitochondrial stress protein.

The EMBO journal ·Vol. 9 ·No. 13 ·1990-12-00 ·Pages 4315-22

Scherer PE, Krieg UC, Hwang ST, Vestweber D, Schatz G

Abstract

We have probed the environment of a precursor protein stuck in mitochondrial import sites using cleavable bifunctional crosslinking reagents. The stuck precursor was crosslinked to a 70 kd protein which, by immunological techniques, was shown to be a matrix protein. The protein was purified to homogeneity by ATP-Sepharose chromatography and partially sequenced. Fourteen of its 15 N-terminal amino acids were identical to residues 24-38 of the protein encoded by the nuclear gene SSC1, which had been proposed to encode a dnaK-like 70 kd mitochondrial stress protein. Our data imply that this mitochondrial hsp70 is made with a cleavable matrix-targeting sequence composed of 23 residues. The complex containing stuck precursor, mitochondrial hsp70, and ISP42 could be solubilized from mitochondria by the non-ionic detergent Triton X-100 even without crosslinking, suggesting tight association of these three components. As the stuck precursor is arrested at an early stage of translocation, mitochondrial hsp70 may initiate the events that lead to refolding of imported precursors in the matrix space.

MeSH Terms
Biological Transport Chimera Cross-Linking Reagents/metabolism Fungal Proteins/immunology Heat-Shock Proteins/immunology,metabolism Membrane Proteins/metabolism Membrane Transport Proteins Microscopy, Immunoelectron Mitochondria/metabolism,ultrastructure Mitochondrial Membrane Transport Proteins Protein Precursors/metabolism Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Saccharomyces cerevisiae Proteins
Chemicals
Cross-Linking Reagents Fungal Proteins Heat-Shock Proteins Membrane Proteins Membrane Transport Proteins Mitochondrial Membrane Transport Proteins Protein Precursors Saccharomyces cerevisiae Proteins Tom40 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Scherer P E
Biocenter, University of Basel, Switzerland.
Krieg U C
Hwang S T
Vestweber D
Schatz G
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-12-00
Pages
4315-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552215
Subset
IM
Grants
NIGMS NIH HHS · 2 R01 GM37803 · United States
External Links
PubMed source PMC Full Text View journal details
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