Mitochondria can import artificial precursor proteins containing a branched polypeptide chain or a carboxy-terminal stilbene disulfonate.
A purified, artificial precursor protein was used as a transport vehicle to test the tolerance of the mitochondrial protein import system. The precursor was a fusion protein consisting of mouse dihydrofolate reductase linked to a yeast mitochondrial presequence; it contained a unique cysteine as its COOH-terminal residue. This COOH-terminal cysteine was covalently coupled to either a stilbene disulfonate derivative or, with the aid of a bifunctional cross-linker, to one of the free amino groups of horse heart cytochrome c. Coupling to horse heart cytochrome c generated a mixture of branched polypeptide chains since this cytochrome lacks a free alpha-amino group. Both adducts were imported and cleaved by isolated yeast mitochondria. The mitochondrial protein import machinery can thus transport more complex structures and even highly charged "membrane-impermeant" organic molecules. This suggests that transport occurs through a hydrophilic environment.
23 references, click to expand
-
A chimeric mitochondrial precursor protein with internal disulfide bridges blocks import of authentic precursors into mitochondria and allows quantitation of import sites.J Cell Biol. 1988 Dec;107(6 Pt 1):2037-43 PMID: 2904445
-
Point mutations destabilizing a precursor protein enhance its post-translational import into mitochondria.EMBO J. 1988 Apr;7(4):1147-51 PMID: 2841113
-
Biogenesis of cytochrome c in Neurospora crassa. Synthesis of apocytochrome c, transfer to mitochondria and conversion to Holocytochrome c.Eur J Biochem. 1978 Nov 15;91(2):609-20 PMID: 215405
-
Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.J Biol Chem. 1982 Nov 10;257(21):13028-33 PMID: 6290489
-
Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.Methods Enzymol. 1983;100:468-500 PMID: 6225933
-
Determinants for protein localization: beta-lactamase signal sequence directs globin across microsomal membranes.Proc Natl Acad Sci U S A. 1984 Jan;81(2):456-60 PMID: 6607473
-
Isolation, characterization and regulation of expression of the nuclear genes for the core II and Rieske iron-sulphur proteins of the yeast ubiquinol-cytochrome c reductase.Gene. 1983 Dec;26(2-3):261-72 PMID: 6323264
-
Insertion of apocytochrome c into lipid vesicles.J Biol Chem. 1984 Apr 10;259(7):4147-56 PMID: 6323460
-
Is the mitochondrial precursor protein apocytochrome c able to pass a lipid barrier?J Biol Chem. 1984 Jun 10;259(11):6704-7 PMID: 6327693
-
Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli.Cell. 1984 Oct;38(3):879-87 PMID: 6386179
-
Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase.Nature. 1985 Jan 31-Feb 6;313(6001):358-63 PMID: 3969146
-
A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.EMBO J. 1984 Dec 20;3(13):3143-8 PMID: 6526014
-
The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.EMBO J. 1984 Dec 20;3(13):3149-56 PMID: 6098467
-
A leader peptide is sufficient to direct mitochondrial import of a chimeric protein.EMBO J. 1985 May;4(5):1129-35 PMID: 3891325
-
Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants.Cell. 1985 Sep;42(2):497-505 PMID: 2992801
-
The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.EMBO J. 1985 Aug;4(8):2061-8 PMID: 2998781
-
Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein.Science. 1986 Feb 21;231(4740):823-9 PMID: 3003913
-
Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria.Nature. 1986 Jul 17-23;322(6076):228-32 PMID: 3016548
-
Interactions of mitochondrial precursor protein apocytochrome c with phosphatidylserine in model membranes. A monolayer study.Biochim Biophys Acta. 1987 Aug 20;902(2):207-16 PMID: 3040096
-
The role of protein structure in the mitochondrial import pathway. Unfolding of mitochondrially bound precursors is required for membrane translocation.J Biol Chem. 1987 Nov 15;262(32):15605-9 PMID: 3680214
-
Mitochondrial precursor proteins are imported through a hydrophilic membrane environment.Eur J Biochem. 1987 Dec 1;169(2):289-93 PMID: 2891506
-
Import of honeybee prepromelittin into the endoplasmic reticulum: energy requirements for membrane insertion.EMBO J. 1988 Mar;7(3):639-48 PMID: 3396536
-
Amino-acid sequence of horse heart cytochrome c.Nature. 1961 Dec 23;192:1125-7 PMID: 14469771
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