Home LiteratureArticle Details
PMID: 2689873 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of mtp70, a mitochondrial member of the hsp70 family.

Molecular and cellular biology ·Vol. 9 ·No. 11 ·1989-11-00 ·Pages 5163-8

Engman DM, Kirchhoff LV, Donelson JE

Abstract

We have isolated a gene from the protozoan parasite Trypanosoma cruzi that encodes a previously unidentified member of the 70-kilodalton heat shock protein (hsp70) family. Among all the eucaryotic hsp70 proteins described to date, this trypanosome protein, mtp70, is uniquely related in sequence and structure to the hsp70 of Escherichia coli, DnaK, which functions in the initiation of DNA replication. This relationship to DnaK is especially relevant in view of the intracellular location of the protein. Within the trypanosome, mtp70 is located in the mitochondrion, where it associates with kinetoplast DNA (kDNA), the unusual mitochondrial DNA that distinguishes this order of protozoa. Moreover, mtp70 is located in the specific region of the kinetoplast in which kDNA replication occurs. In view of the known functions of DnaK, the localization of mtp70 to the site of kDNA replication suggests that mtp70 may participate in eucaryotic mitochondrial DNA replication in a manner analogous to that of DnaK in E. coli.

MeSH Terms
Amino Acid Sequence Animals Blotting, Northern Cloning, Molecular DNA Replication Genes HSP70 Heat-Shock Proteins Heat-Shock Proteins/analysis,genetics Hot Temperature Immune Sera Mitochondria/metabolism Molecular Sequence Data Protozoan Proteins RNA, Messenger/metabolism Sequence Homology, Nucleic Acid Trypanosoma cruzi/analysis,genetics
Chemicals
HSP70 Heat-Shock Proteins Heat-Shock Proteins Immune Sera Protozoan Proteins RNA, Messenger mtp70 protein, Trypanosoma cruzi
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Engman D M
Department of Biochemistry, University of Iowa, Iowa City 52242.
Kirchhoff L V
Donelson J E
References (40)
40 references, click to expand
  1. Plasmodium falciparum gene encoding a protein similar to the 78-kDa rat glucose-regulated stress protein.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6277-81 PMID: 3045815
  2. Ubiquitin genes in trypanosomatidae.
    J Biol Chem. 1988 Sep 5;263(25):12698-704 PMID: 2457589
  3. Protein translocation across membranes.
    Science. 1988 Sep 9;241(4871):1307-13 PMID: 2842866
  4. Structure and expression of the hsp 70 gene family of Leishmania major.
    Nucleic Acids Res. 1988 Oct 25;16(20):9567-85 PMID: 3186441
  5. Replication of kinetoplast DNA in trypanosomes.
    Annu Rev Microbiol. 1988;42:339-58 PMID: 2849370
  6. Localization of a type II DNA topoisomerase to two sites at the periphery of the kinetoplast DNA of Crithidia fasciculata.
    Cell. 1988 Dec 23;55(6):1083-8 PMID: 2849507
  7. Complete nucleotide sequence of the hsp70 gene of T. cruzi.
    Nucleic Acids Res. 1989 Jan 25;17(2):797 PMID: 2644623
  8. The role of stress proteins in membrane biogenesis.
    Trends Biochem Sci. 1988 Oct;13(10):384-8 PMID: 3072700
  9. SSC1, an essential member of the yeast HSP70 multigene family, encodes a mitochondrial protein.
    Mol Cell Biol. 1989 Jul;9(7):3000-8 PMID: 2674677
  10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  11. Replicating, convalently closed, circular DNA from kinetoplasts of Trypanosoma cruzi.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1642-6 PMID: 4556467
  12. Replication of the kinetoplast DNA of Leishmania tarentolae and Crithidia fasciculata.
    Biochim Biophys Acta. 1974 May 17;349(2):161-72 PMID: 4836350
  13. Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum.
    Science. 1976 Feb 27;191(4229):858-9 PMID: 1251198
  14. The replication of kinetoplast DNA networks in Crithidia fasciculata.
    Cell. 1978 May;14(1):157-68 PMID: 667931
  15. Free minicircles of kinetoplast DNA in Crithidia fasciculata.
    J Biol Chem. 1979 Jun 10;254(11):4895-900 PMID: 438220
  16. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  17. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  18. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  19. Has the endosymbiont hypothesis been proven?
    Microbiol Rev. 1982 Mar;46(1):1-42 PMID: 6178009
  20. The dnaK protein of Escherichia coli possesses an ATPase and autophosphorylating activity and is essential in an in vitro DNA replication system.
    Proc Natl Acad Sci U S A. 1983 Nov;80(21):6431-5 PMID: 6314326
  21. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):848-52 PMID: 6322174
  22. Cryptic epitope explains the failure of a monoclonal antibody to bind to certain isolates of Trypanosoma cruzi.
    J Immunol. 1984 Nov;133(5):2731-5 PMID: 6207242
  23. Mitochondrial origins.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4443-7 PMID: 3892535
  24. Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides.
    Mol Cell Biol. 1985 Jun;5(6):1229-37 PMID: 4033650
  25. Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6455-9 PMID: 3931075
  26. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
    EMBO J. 1985 Dec 16;4(13A):3385-91 PMID: 2868889
  27. Uncoating ATPase is a member of the 70 kilodalton family of stress proteins.
    Cell. 1986 Apr 11;45(1):3-13 PMID: 2937542
  28. An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.
    Cell. 1986 Jul 18;46(2):291-300 PMID: 3087629
  29. The heat-shock response.
    Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
  30. Constitutively expressed rat mRNA encoding a 70-kilodalton heat-shock-like protein.
    Mol Cell Biol. 1985 Dec;5(12):3476-83 PMID: 3939319
  31. Speculations on the functions of the major heat shock and glucose-regulated proteins.
    Cell. 1986 Sep 26;46(7):959-61 PMID: 2944601
  32. Cellular and biochemical events in mammalian cells during and after recovery from physiological stress.
    J Cell Biol. 1986 Nov;103(5):2035-52 PMID: 3536957
  33. Conserved sequences and transcription of the hsp70 gene family in Trypanosoma brucei.
    Mol Cell Biol. 1986 Dec;6(12):4657-66 PMID: 3796613
  34. The dnaK gene of Escherichia coli functions in initiation of chromosome replication.
    J Bacteriol. 1988 Feb;170(2):972-9 PMID: 2962992
  35. Heat shock response of the chloroplast genome in Vigna sinensis.
    J Biol Chem. 1988 Apr 15;263(11):5104-9 PMID: 3356681
  36. Heat-shock proteins. Coming in from the cold.
    Nature. 1988 Apr 28;332(6167):776-7 PMID: 3282176
  37. A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides.
    Nature. 1988 Apr 28;332(6167):800-5 PMID: 3282178
  38. 70K heat shock related proteins stimulate protein translocation into microsomes.
    Nature. 1988 Apr 28;332(6167):805-10 PMID: 3282179
  39. Homologous plant and bacterial proteins chaperone oligomeric protein assembly.
    Nature. 1988 May 26;333(6171):330-4 PMID: 2897629
  40. Role of the Escherichia coli DnaK and DnaJ heat shock proteins in the initiation of bacteriophage lambda DNA replication.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6632-6 PMID: 2970643
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.11.5163-5168.1989
Published
1989-11-00
Pages
5163-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363668
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.11.5163-5168.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2