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PMID: 4556467 Published · ppublish English Journal Article

Replicating, convalently closed, circular DNA from kinetoplasts of Trypanosoma cruzi.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 6 ·1972-06-00 ·Pages 1642-6

Brack C, Delain E, Riou G

Abstract

When Trypanosoma cruzi are treated with Berenil, a trypanocide, their kinetoplast DNA contains an increased proportion of double-branched circular molecules. These replicating molecules have closed-circular template strands; their decrease in density when complexed by ethidium bromide in a cesium chloride gradient is proportional to the length of the replicated segments. Replication seems to be blocked at specific points, which are equidistantly spaced along the circular kinetoplast DNA molecules. Analysis of about 800 replicating forms showed that the lengths of the replicated branches are not distributed at random, but into several populations, which correspond to multiples of 15% of the total contour length of 0.5 mum. This distribution evokes a discontinuous replication process. The problem of whether kinetoplast DNA is synthesized by successive replication units, or whether and how Berenil might induce specific blocking of DNA replication, is discussed.

MeSH Terms
Acetates/pharmacology Amidines/pharmacology Antiprotozoal Agents/pharmacology Centrifugation, Density Gradient DNA/analysis DNA Replication/drug effects Ethidium/pharmacology Glycine/pharmacology Microscopy, Electron Mitochondria/analysis Nucleic Acid Conformation Triazenes/pharmacology Trypanosoma cruzi/analysis,cytology,drug effects
Chemicals
Acetates Amidines Antiprotozoal Agents Triazenes DNA Ethidium Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brack C
Delain E
Riou G
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.6.1642
Published
1972-06-00
Pages
1642-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426766
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.6.1642 PMC Full Text View journal details
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