Home LiteratureArticle Details
PMID: 3939319 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Constitutively expressed rat mRNA encoding a 70-kilodalton heat-shock-like protein.

Molecular and cellular biology ·Vol. 5 ·No. 12 ·1985-12-00 ·Pages 3476-83

O'Malley K, Mauron A, Barchas JD, Kedes L

Abstract

A nearly full-length cDNA clone isolated from the rat pheochromocytoma cell line, PC12, revealed extensive nucleotide sequence similarity between the rat cDNA and the Drosophila melanogaster hsp70 gene. The rat recombinant clone encodes a 71,000-dalton protein that is 70% identical with the dipteran hsp70 protein. Remarkably, a truncated segment of this cDNA clone was originally isolated by immunoreactivity with antisera raised to catecholamine-synthesizing enzymes, suggesting that this heat shock protein and these catecholamine enzymes shared antigenic determinants. The rat hsp70-related mRNA is responsible for the production of a constitutive hsp70 protein, because it is present in abundant amounts in various tissues at normal growth temperatures and is only minimally induced by hyperthermia. The rat hsp70-related sequence is part of a multigene family that extends across species to mice and humans.

MeSH Terms
Animals Base Sequence Cell Line DNA/genetics Drosophila melanogaster/genetics Gene Expression Regulation Heat-Shock Proteins/biosynthesis,genetics Humans Mice Nucleic Acid Hybridization RNA, Messenger/genetics Rats Species Specificity Tissue Distribution
Chemicals
Heat-Shock Proteins RNA, Messenger DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
O'Malley K
Mauron A
Barchas J D
Kedes L
References (31)
31 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions.
    Cell. 1980 Oct;21(3):669-79 PMID: 6777045
  3. Complete coding sequence of rat tyrosine hydroxylase mRNA.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):617-21 PMID: 2857492
  4. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  5. Cellular responses to stress: comparison of a family of 71--73-kilodalton proteins rapidly synthesized in rat tissue slices and canavanine-treated cells in culture.
    J Cell Physiol. 1981 Aug;108(2):261-75 PMID: 7263772
  6. Dopamine beta-hydroxylase rat mRNA: structure, regulation, and tissue localization.
    Cold Spring Harb Symp Quant Biol. 1983;48 Pt 1:319-25 PMID: 6144415
  7. Drosophila gene related to the major heat shock-induced gene is transcribed at normal temperatures and not induced by heat shock.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):525-9 PMID: 6804941
  8. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  9. Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells.
    J Biol Chem. 1984 Apr 10;259(7):4501-13 PMID: 6368558
  10. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  11. Heat shock response of Dictyostelium.
    Dev Biol. 1980 Oct;79(2):399-408 PMID: 6775994
  12. The effect of amino acid analogues and heat shock on gene expression in chicken embryo fibroblasts.
    Cell. 1978 Dec;15(4):1277-86 PMID: 569556
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila.
    Mol Cell Biol. 1982 Nov;2(11):1388-98 PMID: 6761581
  15. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
    Mol Cell Biol. 1983 May;3(5):787-95 PMID: 6865942
  16. Genes for catecholamine biosynthesis: cloning by expression and identification of the cDNA for rat dopamine beta-hydroxylase.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2161-5 PMID: 6572968
  17. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.
    Cell. 1980 Aug;21(1):179-87 PMID: 6157477
  18. A response of protein synthesis to temperature shift in the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5222-5 PMID: 388432
  19. Studies of cloned sequences from four Drosophila heat shock loci.
    Cell. 1979 Dec;18(4):1359-70 PMID: 117907
  20. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):848-52 PMID: 6322174
  21. 70-Kilodalton heat shock polypeptides from rainbow trout: characterization of cDNA sequences.
    Mol Cell Biol. 1984 Sep;4(9):1785-91 PMID: 6092938
  22. Heat shock proteins of higher plants.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3526-30 PMID: 16593032
  23. Antibodies to two major chicken heat shock proteins cross-react with similar proteins in widely divergent species.
    Mol Cell Biol. 1982 Mar;2(3):267-74 PMID: 7110134
  24. Hsp70 accelerates the recovery of nucleolar morphology after heat shock.
    EMBO J. 1984 Dec 20;3(13):3095-100 PMID: 6441707
  25. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments.
    Chromosoma. 1970;31(3):356-76 PMID: 5489366
  26. The induction of gene activity in drosophilia by heat shock.
    Cell. 1979 Jun;17(2):241-54 PMID: 110462
  27. Evidence for the existence of homologous gene coding regions for the catecholamine biosynthetic enzymes.
    Cold Spring Harb Symp Quant Biol. 1983;48 Pt 1:327-35 PMID: 6144416
  28. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  29. Mouse and Drosophila genes encoding the major heat shock protein (hsp70) are highly conserved.
    Mol Cell Biol. 1983 Aug;3(8):1540-3 PMID: 6413838
  30. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  31. Developmental control of the heat shock response in Xenopus.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3138-42 PMID: 6203112
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.12.3476-3483.1985
Published
1985-12-00
Pages
3476-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369178
Subset
IM
Grants
NIMH NIH HHS · MH23861 · United States
Databases
GENBANK
M11942
External Links
PubMed source DOI: 10.1128/mcb.5.12.3476-3483.1985 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2