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PMID: 6098449 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

The EMBO journal ·Vol. 3 ·No. 12 ·1984-12-01 ·Pages 2831-7

Maarse AC, Van Loon AP, Riezman H, Gregor I, Schatz G, Grivell LA

Abstract

The six small subunits (IV-VII, VIIa, VIII) of yeast cytochrome c oxidase are encoded by nuclear genes and imported into the mitochondria. We have isolated the gene for subunit IV from a yeast genomic clone bank and determined its complete nucleotide sequence. We have also isolated subunit IV from purified yeast cytochrome c oxidase and determined most of its amino acid sequence which confirms the positioning of approximately 90% of the amino acid residues. The sequence comparison shows that the coding sequence of the gene lacks introns and that subunit IV is made as a precursor with an amino-terminal extension of 25 residues, five of which are basic and none of them acidic. Precursor processing involves cleavage of a Leu-Gln bond.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA Restriction Enzymes Electron Transport Complex IV/genetics Genes Genes, Fungal Macromolecular Substances Mitochondria/enzymology Neurospora crassa/enzymology,genetics Plasmids Saccharomyces cerevisiae/enzymology,genetics Species Specificity
Chemicals
Macromolecular Substances Electron Transport Complex IV DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Maarse A C
Van Loon A P
Riezman H
Gregor I
Schatz G
Grivell L A
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47 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-12-01
Pages
2831-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557773
Subset
IM
Databases
GENBANK
X01418
External Links
PubMed source PMC Full Text View journal details
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