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PMID: 15725353 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Review

The Vpr protein from HIV-1: distinct roles along the viral life cycle.

Retrovirology ·Vol. 2 ·2005-02-22 ·Pages 11

Le Rouzic E, Benichou S

Abstract

The genomes of human and simian immunodeficiency viruses (HIV and SIV) encode the gag, pol and env genes and contain at least six supplementary open reading frames termed tat, rev, nef, vif, vpr, vpx and vpu. While the tat and rev genes encode regulatory proteins absolutely required for virus replication, nef, vif, vpr, vpx and vpu encode for small proteins referred to "auxiliary" (or "accessory"), since their expression is usually dispensable for virus growth in many in vitro systems. However, these auxiliary proteins are essential for viral replication and pathogenesis in vivo. The two vpr- and vpx-related genes are found only in members of the HIV-2/SIVsm/SIVmac group, whereas primate lentiviruses from other lineages (HIV-1, SIVcpz, SIVagm, SIVmnd and SIVsyk) contain a single vpr gene. In this review, we will mainly focus on vpr from HIV-1 and discuss the most recent developments in our understanding of Vpr functions and its role during the virus replication cycle.

MeSH Terms
Gene Products, vpr/chemistry,genetics,metabolism Genes, vpr HIV-1/genetics,pathogenicity,physiology Humans Models, Molecular Virus Replication vpr Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, vpr vpr Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Le Rouzic Erwann
Institut Cochin, Department of Infectious Diseases, INSERM U567, CNRS UMR8104, Université Paris 5, Paris, France. lerouzic@cochin.inserm.fr
Benichou Serge
References (181)
181 references, click to expand
  1. Checking out the G(2)/M transition.
    Biochim Biophys Acta. 2001 May 28;1519(1-2):1-12 PMID: 11406266
  2. T cell apoptosis causes peripheral T cell depletion in mice transgenic for the HIV-1 vpr gene.
    Virology. 2001 Jul 5;285(2):181-92 PMID: 11437653
  3. Journey to the center of the cell.
    Cell. 2001 Jun 15;105(6):697-700 PMID: 11440710
  4. Nuclear export of human immunodeficiency virus type 1 Vpr is not required for virion packaging.
    J Virol. 2001 Sep;75(17):8348-52 PMID: 11483780
  5. HIV-1 Vpr transactivates LTR-directed expression through sequences present within -278 to -176 and increases virus replication in vitro.
    Virology. 2001 Oct 25;289(2):334-42 PMID: 11689055
  6. Virology. HIV--breaking the rules for nuclear entry.
    Science. 2001 Nov 2;294(5544):1016-7 PMID: 11691977
  7. Dynamic disruptions in nuclear envelope architecture and integrity induced by HIV-1 Vpr.
    Science. 2001 Nov 2;294(5544):1105-8 PMID: 11691994
  8. The expanding role of mitochondria in apoptosis.
    Genes Dev. 2001 Nov 15;15(22):2922-33 PMID: 11711427
  9. HIV-1 Vpr enhances viral burden by facilitating infection of tissue macrophages but not nondividing CD4+ T cells.
    J Exp Med. 2001 Nov 19;194(10):1407-19 PMID: 11714748
  10. Intracellular trafficking of retroviral genomes during the early phase of infection: viral exploitation of cellular pathways.
    J Gene Med. 2001 Nov-Dec;3(6):517-28 PMID: 11778899
  11. S phase and G2 arrests induced by topoisomerase I poisons are dependent on ATR kinase function.
    J Biol Chem. 2002 Jan 11;277(2):1599-606 PMID: 11700302
  12. Slipping through the door: HIV entry into the nucleus.
    Microbes Infect. 2002 Jan;4(1):67-73 PMID: 11825777
  13. Phosphorylation of Vpr regulates HIV type 1 nuclear import and macrophage infection.
    AIDS Res Hum Retroviruses. 2002 Mar 1;18(4):283-8 PMID: 11860675
  14. Lentiviral expression of HIV-1 Vpr induces apoptosis in human neurons.
    J Neurovirol. 2002 Apr;8(2):86-99 PMID: 11935461
  15. NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol. Comparison with the Vpr N-terminal (1-51) and C-terminal (52-96) domains.
    Eur J Biochem. 2002 Aug;269(15):3779-88 PMID: 12153575
  16. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein.
    Nature. 2002 Aug 8;418(6898):646-50 PMID: 12167863
  17. Human immunodeficiency virus type 1 (HIV-1) accessory protein Vpr induces transcription of the HIV-1 and glucocorticoid-responsive promoters by binding directly to p300/CBP coactivators.
    J Virol. 2002 Oct;76(19):9724-34 PMID: 12208951
  18. Proteomic analysis of the mammalian nuclear pore complex.
    J Cell Biol. 2002 Sep 2;158(5):915-27 PMID: 12196509
  19. Roles of uracil-DNA glycosylase and dUTPase in virus replication.
    J Gen Virol. 2002 Oct;83(Pt 10):2339-45 PMID: 12237414
  20. HIV-1 Vpr induces apoptosis through caspase 9 in T cells and peripheral blood mononuclear cells.
    J Biol Chem. 2002 Oct 4;277(40):37820-31 PMID: 12095993
  21. The C-terminal moiety of HIV-1 Vpr induces cell death via a caspase-independent mitochondrial pathway.
    Cell Death Differ. 2002 Nov;9(11):1212-9 PMID: 12404120
  22. Lamins: building blocks or regulators of gene expression?
    Nat Rev Mol Cell Biol. 2002 Nov;3(11):848-58 PMID: 12415302
  23. Visualization of the intracellular behavior of HIV in living cells.
    J Cell Biol. 2002 Nov 11;159(3):441-52 PMID: 12417576
  24. Vpr-mediated incorporation of UNG2 into HIV-1 particles is required to modulate the virus mutation rate and for replication in macrophages.
    J Biol Chem. 2004 Jul 2;279(27):28419-25 PMID: 15096517
  25. Mutational analysis of the human immunodeficiency virus vpr open reading frame.
    J Virol. 1989 Sep;63(9):4110-4 PMID: 2474678
  26. Human immunodeficiency virus vpr product is a virion-associated regulatory protein.
    J Virol. 1990 Jun;64(6):3097-9 PMID: 2139896
  27. Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex.
    J Virol. 1991 Apr;65(4):1910-5 PMID: 2002549
  28. Human immunodeficiency virus type 1 viral protein R localization in infected cells and virions.
    J Virol. 1993 Nov;67(11):6542-50 PMID: 8411357
  29. A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells.
    Nature. 1993 Oct 14;365(6447):666-9 PMID: 8105392
  30. The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells.
    Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7311-5 PMID: 8041786
  31. Serum Vpr regulates productive infection and latency of human immunodeficiency virus type 1.
    Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10873-7 PMID: 7971975
  32. Biochemical mechanism of HIV-1 Vpr function. Oligomerization mediated by the N-terminal domain.
    J Biol Chem. 1994 Dec 23;269(51):32131-7 PMID: 7798208
  33. Extracellular Vpr protein increases cellular permissiveness to human immunodeficiency virus replication and reactivates virus from latency.
    J Virol. 1995 Feb;69(2):1243-52 PMID: 7815499
  34. Vpr is required for efficient replication of human immunodeficiency virus type-1 in mononuclear phagocytes.
    Virology. 1995 Feb 1;206(2):935-44 PMID: 7531918
  35. Progression to AIDS in the absence of a gene for vpr or vpx.
    J Virol. 1995 Apr;69(4):2378-83 PMID: 7884883
  36. The glucocorticoid receptor type II complex is a target of the HIV-1 vpr gene product.
    Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3621-5 PMID: 7724608
  37. Targeting foreign proteins to human immunodeficiency virus particles via fusion with Vpr and Vpx.
    J Virol. 1995 Jun;69(6):3389-98 PMID: 7745685
  38. vpr deletion mutant of simian immunodeficiency virus induces AIDS in rhesus monkeys.
    J Virol. 1995 Aug;69(8):4807-13 PMID: 7609047
  39. Lower in vivo mutation rate of human immunodeficiency virus type 1 than that predicted from the fidelity of purified reverse transcriptase.
    J Virol. 1995 Aug;69(8):5087-94 PMID: 7541846
  40. The human immunodeficiency virus type 1 vpr gene arrests infected T cells in the G2 + M phase of the cell cycle.
    J Virol. 1995 Oct;69(10):6304-13 PMID: 7666531
  41. Human immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of the cell cycle by inhibiting p34cdc2 activity.
    J Virol. 1995 Nov;69(11):6705-11 PMID: 7474080
  42. Human immunodeficiency virus type 1 Vpr arrests the cell cycle in G2 by inhibiting the activation of p34cdc2-cyclin B.
    J Virol. 1995 Nov;69(11):6859-64 PMID: 7474100
  43. Mutagenic analysis of human immunodeficiency virus type 1 Vpr: role of a predicted N-terminal alpha-helical structure in Vpr nuclear localization and virion incorporation.
    J Virol. 1995 Nov;69(11):7032-44 PMID: 7474123
  44. Interaction of virion protein Vpr of human immunodeficiency virus type 1 with cellular transcription factor Sp1 and trans-activation of viral long terminal repeat.
    J Biol Chem. 1995 Oct 27;270(43):25564-9 PMID: 7592727
  45. Mutational analysis of cell cycle arrest, nuclear localization and virion packaging of human immunodeficiency virus type 1 Vpr.
    J Virol. 1995 Dec;69(12):7909-16 PMID: 7494303
  46. A conserved LXXLF sequence is the major determinant in p6gag required for the incorporation of human immunodeficiency virus type 1 Vpr.
    J Virol. 1996 Jan;70(1):159-64 PMID: 8523520
  47. Vpr protein of human immunodeficiency virus type 1 forms cation-selective channels in planar lipid bilayers.
    Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):111-5 PMID: 8552585
  48. Human immunodeficiency virus type 1 Vpr protein binds to the uracil DNA glycosylase DNA repair enzyme.
    J Virol. 1996 Feb;70(2):697-704 PMID: 8551605
  49. Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import.
    J Virol. 1996 Feb;70(2):1027-32 PMID: 8551560
  50. A conserved dileucine-containing motif in p6(gag) governs the particle association of Vpx and Vpr of simian immunodeficiency viruses SIV(mac) and SIV(agm).
    J Virol. 1999 Dec;73(12):9992-9 PMID: 10559313
  51. A novel nuclear export activity in HIV-1 matrix protein required for viral replication.
    Nature. 1999 Dec 9;402(6762):681-5 PMID: 10604476
  52. The HIV-1 viral protein R induces apoptosis via a direct effect on the mitochondrial permeability transition pore.
    J Exp Med. 2000 Jan 3;191(1):33-46 PMID: 10620603
  53. Biochemical and structural analysis of isolated mature cores of human immunodeficiency virus type 1.
    J Virol. 2000 Feb;74(3):1168-77 PMID: 10627527
  54. HIV-1 genome nuclear import is mediated by a central DNA flap.
    Cell. 2000 Apr 14;101(2):173-85 PMID: 10786833
  55. Efficient DNA transfection mediated by the C-terminal domain of human immunodeficiency virus type 1 viral protein R.
    J Virol. 2000 Jun;74(12):5424-31 PMID: 10823846
  56. A carboxy-terminally truncated form of the human immunodeficiency virus type 1 Vpr protein induces apoptosis via G(1) cell cycle arrest.
    J Virol. 2000 Jul;74(13):6058-67 PMID: 10846089
  57. Isolation of human immunodeficiency virus type 1 cores: retention of Vpr in the absence of p6(gag).
    J Virol. 2000 Jul;74(13):6198-202 PMID: 10846106
  58. Interactions of the C-terminus of viral protein R with nucleic acids are modulated by its N-terminus.
    Eur J Biochem. 2000 Jun;267(12):3654-60 PMID: 10848983
  59. Two nuclear localization signals in the HIV-1 matrix protein regulate nuclear import of the HIV-1 pre-integration complex.
    J Mol Biol. 2000 Jun 2;299(2):359-68 PMID: 10860744
  60. Phosphorylation of human immunodeficiency virus type 1 Vpr regulates cell cycle arrest.
    J Virol. 2000 Jul;74(14):6520-7 PMID: 10864665
  61. Morphologic changes in human immunodeficiency virus type 1 virions secondary to intravirion reverse transcription: evidence indicating that reverse transcription may not take place within the intact viral core.
    J Hum Virol. 2000 May-Jun;3(3):165-72 PMID: 10881997
  62. Effects of extracellular human immunodeficiency virus type 1 vpr protein in primary rat cortical cell cultures.
    J Neurovirol. 2000 Jun;6(3):202-20 PMID: 10878710
  63. The interaction of vpr with uracil DNA glycosylase modulates the human immunodeficiency virus type 1 In vivo mutation rate.
    J Virol. 2000 Aug;74(15):7039-47 PMID: 10888643
  64. Two putative alpha-helical domains of human immunodeficiency virus type 1 Vpr mediate nuclear localization by at least two mechanisms.
    J Virol. 2000 Aug;74(15):7179-86 PMID: 10888660
  65. Induction of M-phase arrest and apoptosis after HIV-1 Vpr expression through uncoupling of nuclear and centrosomal cycle in HeLa cells.
    Exp Cell Res. 2000 Aug 1;258(2):261-9 PMID: 10896777
  66. Human immunodeficiency virus type 1 Vpr-mediated G(2) cell cycle arrest: Vpr interferes with cell cycle signaling cascades by interacting with the B subunit of serine/threonine protein phosphatase 2A.
    EMBO J. 2000 Aug 1;19(15):3956-67 PMID: 10921877
  67. Human immunodeficiency virus type 1 Vpr contains two leucine-rich helices that mediate glucocorticoid receptor coactivation independently of its effects on G(2) cell cycle arrest.
    J Virol. 2000 Sep;74(17):8159-65 PMID: 10933727
  68. Heat-shock protein 70 can replace viral protein R of HIV-1 during nuclear import of the viral preintegration complex.
    Exp Cell Res. 2000 Sep 15;259(2):398-403 PMID: 10964507
  69. Human immunodeficiency virus type 1 Vpr induces apoptosis in human neuronal cells.
    J Virol. 2000 Oct;74(20):9717-26 PMID: 11000244
  70. Human immunodeficiency virus type 1 Vpr protein is incorporated into the virion in significantly smaller amounts than gag and is phosphorylated in infected cells.
    J Virol. 2000 Oct;74(20):9727-31 PMID: 11000245
  71. Type 2A protein phosphatase, the complex regulator of numerous signaling pathways.
    Biochem Pharmacol. 2000 Oct 15;60(8):1225-35 PMID: 11007961
  72. Induction of apoptosis by the Vpr protein of human immunodeficiency virus type 1 occurs independently of G(2) arrest of the cell cycle.
    Virology. 2000 Oct 10;276(1):16-26 PMID: 11021990
  73. Cellular distribution and karyophilic properties of matrix, integrase, and Vpr proteins from the human and simian immunodeficiency viruses.
    Exp Cell Res. 2000 Nov 1;260(2):387-95 PMID: 11035935
  74. Functional and structural characterization of synthetic HIV-1 Vpr that transduces cells, localizes to the nucleus, and induces G2 cell cycle arrest.
    J Biol Chem. 2000 Oct 13;275(41):32016-26 PMID: 10903315
  75. Functional role of residues corresponding to helical domain II (amino acids 35 to 46) of human immunodeficiency virus type 1 Vpr.
    J Virol. 2000 Nov;74(22):10650-7 PMID: 11044109
  76. Viral replication and the coactivators p300 and CBP.
    Trends Microbiol. 2000 Dec;8(12):560-5 PMID: 11115752
  77. Nucleocytoplasmic shuttling by human immunodeficiency virus type 1 Vpr.
    J Virol. 2001 Feb;75(3):1522-32 PMID: 11152524
  78. HHR23A, the human homologue of the yeast repair protein RAD23, interacts specifically with Vpr protein and prevents cell cycle arrest but not the transcriptional effects of Vpr.
    Virology. 1998 Jun 5;245(2):323-30 PMID: 9636371
  79. Cell cycle arrest by Vpr in HIV-1 virions and insensitivity to antiretroviral agents.
    Science. 1998 Jul 10;281(5374):266-9 PMID: 9657723
  80. A peptide derived from the N-terminal region of HIV-1 Vpr promotes nuclear import in permeabilized cells: elucidation of the NLS region of the Vpr.
    FEBS Lett. 1998 Jun 16;429(3):421-5 PMID: 9662462
  81. Cooperative actions of HIV-1 Vpr and p53 modulate viral gene transcription.
    J Biol Chem. 1998 Aug 7;273(32):20052-7 PMID: 9685344
  82. Characterization of HIV-1 vpr nuclear import: analysis of signals and pathways.
    J Cell Biol. 1998 Nov 16;143(4):875-85 PMID: 9817747
  83. Establishment of a functional human immunodeficiency virus type 1 (HIV-1) reverse transcription complex involves the cytoskeleton.
    J Exp Med. 1998 Dec 7;188(11):2113-25 PMID: 9841925
  84. Vpx is required for dissemination and pathogenesis of SIV(SM) PBj: evidence of macrophage-dependent viral amplification.
    Nat Med. 1998 Dec;4(12):1401-8 PMID: 9846578
  85. Incorporation of Vpr into human immunodeficiency virus type 1 requires a direct interaction with the p6 domain of the p55 gag precursor.
    J Biol Chem. 1999 Mar 26;274(13):9083-91 PMID: 10085158
  86. Human immunodeficiency virus type 1 (HIV-1) Vpr functions as an immediate-early protein during HIV-1 infection.
    J Virol. 1999 May;73(5):4101-9 PMID: 10196306
  87. The amino-terminal region of Vpr from human immunodeficiency virus type 1 forms ion channels and kills neurons.
    J Virol. 1999 May;73(5):4230-8 PMID: 10196319
  88. The HIV-1 Vpr co-activator induces a conformational change in TFIIB.
    FEBS Lett. 1999 May 7;450(3):235-9 PMID: 10359081
  89. Cell cycle- and Vpr-mediated regulation of human immunodeficiency virus type 1 expression in primary and transformed T-cell lines.
    J Virol. 1999 Jul;73(7):5422-30 PMID: 10364289
  90. Viral protein R of HIV-1.
    Rev Med Virol. 1999 Jan-Mar;9(1):39-49 PMID: 10371671
  91. Nuclear import of human immunodeficiency virus type-1 preintegration complexes.
    Adv Virus Res. 1999;52:275-99 PMID: 10384238
  92. Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal.
    Curr Biol. 1999 Jul 1;9(13):680-9 PMID: 10395539
  93. Targeting human immunodeficiency virus (HIV) type 2 integrase protein into HIV type 1.
    J Virol. 1999 Oct;73(10):8831-6 PMID: 10482639
  94. HIV-1 Vpr-chloramphenicol acetyltransferase fusion proteins: sequence requirement for virion incorporation and analysis of antiviral effect.
    Gene Ther. 1999 Sep;6(9):1590-9 PMID: 10490769
  95. The C-terminal domain of the HIV-1 regulatory protein Vpr adopts an antiparallel dimeric structure in solution via its leucine-zipper-like domain.
    Biochem J. 2005 Apr 15;387(Pt 2):333-41 PMID: 15571493
  96. HIV-1 Vpr displays natural protein-transducing properties: implications for viral pathogenesis.
    Virology. 2002 Oct 10;302(1):95-105 PMID: 12429519
  97. Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1.
    J Biol Chem. 2002 Nov 22;277(47):45091-8 PMID: 12228227
  98. Carboxyl terminus of hVIP/mov34 is critical for HIV-1-Vpr interaction and glucocorticoid-mediated signaling.
    J Biol Chem. 2002 Dec 6;277(49):47854-60 PMID: 12237292
  99. Genetic selection of peptide inhibitors of human immunodeficiency virus type 1 Vpr.
    J Biol Chem. 2002 Dec 13;277(50):48816-26 PMID: 12379652
  100. Upregulation of survivin by HIV-1 Vpr.
    Apoptosis. 2003 Jan;8(1):71-9 PMID: 12510154
  101. Depletion of Wee-1 kinase is necessary for both human immunodeficiency virus type 1 Vpr- and gamma irradiation-induced apoptosis.
    J Virol. 2003 Feb;77(3):2063-70 PMID: 12525641
  102. HIV-1 Vpr activates cell cycle inhibitor p21/Waf1/Cip1: a potential mechanism of G2/M cell cycle arrest.
    Virology. 2003 Jan 20;305(2):371-7 PMID: 12573582
  103. Incorporation of uracil into minus strand DNA affects the specificity of plus strand synthesis initiation during lentiviral reverse transcription.
    J Biol Chem. 2003 Mar 7;278(10):7902-9 PMID: 12458216
  104. NMR structure of the HIV-1 regulatory protein VPR.
    J Mol Biol. 2003 Mar 14;327(1):215-27 PMID: 12614620
  105. Human immunodeficiency virus type 1 (HIV-1) Vpr enhances expression from unintegrated HIV-1 DNA.
    J Virol. 2003 Apr;77(7):3962-72 PMID: 12634356
  106. HIV-1 Vpr binding to HIV-1 LTR C/EBP cis-acting elements and adjacent regions is sequence-specific.
    Biomed Pharmacother. 2003 Jan;57(1):41-8 PMID: 12642036
  107. Nuclear import of viral DNA genomes.
    Traffic. 2003 Mar;4(3):136-43 PMID: 12656986
  108. Specific G2 arrest of caprine cells infected with a caprine arthritis encephalitis virus expressing vpr and vpx genes from simian immunodeficiency virus.
    Virology. 2003 Apr 25;309(1):41-52 PMID: 12726725
  109. Mechanism of HIV-1 viral protein R-induced apoptosis.
    Biochem Biophys Res Commun. 2003 May 9;304(3):583-92 PMID: 12729593
  110. The cationic amphipathic alpha-helix of HIV-1 viral protein R (Vpr) binds to nucleic acids, permeabilizes membranes, and efficiently transfects cells.
    J Biol Chem. 2003 May 16;278(20):18110-6 PMID: 12639957
  111. Current strategies in the treatment of HIV infection.
    Adv Intern Med. 2001;46:207-46 PMID: 11147254
  112. NMR structure of the (1-51) N-terminal domain of the HIV-1 regulatory protein Vpr.
    Eur J Biochem. 1999 Dec;266(2):359-69 PMID: 10561576
  113. Control of mitochondrial membrane permeabilization by adenine nucleotide translocator interacting with HIV-1 viral protein rR and Bcl-2.
    J Exp Med. 2001 Feb 19;193(4):509-19 PMID: 11181702
  114. Interaction of human immunodeficiency virus type 1 Vpr with the HHR23A DNA repair protein does not correlate with multiple biological functions of Vpr.
    Virology. 2001 Mar 30;282(1):176-85 PMID: 11259200
  115. Characterization of intracellular reverse transcription complexes of human immunodeficiency virus type 1.
    J Virol. 2001 Apr;75(8):3626-35 PMID: 11264352
  116. Apoptosis in AIDS.
    Apoptosis. 2001 Feb-Apr;6(1-2):103-16 PMID: 11321033
  117. The nuclear pore complex as a transport machine.
    J Biol Chem. 2001 May 18;276(20):16593-6 PMID: 11283009
  118. The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB.
    J Mol Biol. 1996 Sep 6;261(5):599-606 PMID: 8800208
  119. The mutation rate of human immunodeficiency virus type 1 is influenced by the vpr gene.
    Virology. 1996 Aug 15;222(2):391-400 PMID: 8806523
  120. Characterization of a leucine-zipper-like domain in Vpr protein of human immunodeficiency virus type 1.
    Gene. 1996 Oct 31;178(1-2):7-13 PMID: 8921884
  121. Human immunodeficiency virus type 1 vpr gene induces phenotypic effects similar to those of the DNA alkylating agent, nitrogen mustard.
    J Virol. 1997 May;71(5):3961-71 PMID: 9094673
  122. The HIV-1 virion-associated protein vpr is a coactivator of the human glucocorticoid receptor.
    J Exp Med. 1999 Jan 4;189(1):51-62 PMID: 9874563
  123. Interaction with the p6 domain of the gag precursor mediates incorporation into virions of Vpr and Vpx proteins from primate lentiviruses.
    J Virol. 1999 Jan;73(1):592-600 PMID: 9847364
  124. NMR structure of the (52-96) C-terminal domain of the HIV-1 regulatory protein Vpr: molecular insights into its biological functions.
    J Mol Biol. 1999 Feb 5;285(5):2105-17 PMID: 9925788
  125. Human immunodeficiency virus type 1 integrase protein promotes reverse transcription through specific interactions with the nucleoprotein reverse transcription complex.
    J Virol. 1999 Mar;73(3):2126-35 PMID: 9971795
  126. Vpr R77Q is associated with long-term nonprogressive HIV infection and impaired induction of apoptosis.
    J Clin Invest. 2003 May;111(10):1547-54 PMID: 12750404
  127. Hypermutation of HIV-1 DNA in the absence of the Vif protein.
    Science. 2003 May 16;300(5622):1112 PMID: 12750511
  128. Recruitment of HIV and its receptors to dendritic cell-T cell junctions.
    Science. 2003 May 23;300(5623):1295-7 PMID: 12730499
  129. Molecular biology. Beginning at the end.
    Science. 2003 Jun 6;300(5625):1512-3 PMID: 12791969
  130. Nuclear export of Vpr is required for efficient replication of human immunodeficiency virus type 1 in tissue macrophages.
    J Virol. 2003 Jul;77(13):7582-9 PMID: 12805458
  131. Mechanisms of CD4+ T lymphocyte cell death in human immunodeficiency virus infection and AIDS.
    J Gen Virol. 2003 Jul;84(Pt 7):1649-61 PMID: 12810858
  132. The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA.
    Nature. 2003 Jul 3;424(6944):94-8 PMID: 12808465
  133. Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts.
    Nature. 2003 Jul 3;424(6944):99-103 PMID: 12808466
  134. Activation of the ATR-mediated DNA damage response by the HIV-1 viral protein R.
    J Biol Chem. 2003 Jul 11;278(28):25879-86 PMID: 12738771
  135. Nuclear import of HIV-1 intracellular reverse transcription complexes is mediated by importin 7.
    EMBO J. 2003 Jul 15;22(14):3675-85 PMID: 12853482
  136. Structural analyses of purified human immunodeficiency virus type 1 intracellular reverse transcription complexes.
    J Virol. 2003 Aug;77(15):8196-206 PMID: 12857888
  137. Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif.
    Cell. 2003 Jul 11;114(1):21-31 PMID: 12859895
  138. Studies with GFP-Vpr fusion proteins: induction of apoptosis but ablation of cell-cycle arrest despite nuclear membrane or nuclear localization.
    Virology. 2003 Aug 15;313(1):91-104 PMID: 12951024
  139. Replication of chimeric human immunodeficiency virus type 1 (HIV-1) containing HIV-2 integrase (IN): naturally selected mutations in IN augment DNA synthesis.
    J Virol. 2003 Oct;77(20):11050-9 PMID: 14512553
  140. Cyclophilin A interacts with HIV-1 Vpr and is required for its functional expression.
    J Biol Chem. 2003 Oct 31;278(44):43202-13 PMID: 12881522
  141. Structural characterization of the HIV-1 Vpr N terminus: evidence of cis/trans-proline isomerism.
    J Biol Chem. 2003 Oct 31;278(44):43188-201 PMID: 12881523
  142. Pro-apoptotic activity of HIV-1 auxiliary regulatory protein Vpr is subtype-dependent and potently enhanced by nonconservative changes of the leucine residue at position 64.
    J Biol Chem. 2003 Nov 7;278(45):44326-30 PMID: 14506268
  143. Differential regulation of host cellular genes by HIV-1 viral protein R (Vpr): cDNA microarray analysis using isogenic virus.
    Biochem Biophys Res Commun. 2004 Feb 20;314(4):1126-32 PMID: 14751250
  144. HIV-1 Vpr induces defects in mitosis, cytokinesis, nuclear structure, and centrosomes.
    Mol Biol Cell. 2004 Apr;15(4):1793-801 PMID: 14767062
  145. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control.
    J Virol. 1996 Apr;70(4):2324-31 PMID: 8642659
  146. Vpr-induced cell cycle arrest is conserved among primate lentiviruses.
    J Virol. 1996 Apr;70(4):2516-24 PMID: 8642681
  147. Evidence for direct association of Vpr and matrix protein p17 within the HIV-1 virion.
    Virology. 1996 Jun 1;220(1):208-12 PMID: 8659115
  148. Effect of human immunodeficiency virus type 1 protein R (vpr) gene expression on basic cellular function of fission yeast Schizosaccharomyces pombe.
    J Virol. 1996 Sep;70(9):5821-6 PMID: 8709199
  149. Mutations of p34cdc2 phosphorylation sites induce premature mitotic events in HeLa cells: evidence for a double block to p34cdc2 kinase activation in vertebrates.
    EMBO J. 1991 Nov;10(11):3331-41 PMID: 1655418
  150. Evolution of the primate lentiviruses: evidence from vpx and vpr.
    EMBO J. 1992 Sep;11(9):3405-12 PMID: 1324171
  151. Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus.
    J Virol. 1993 Feb;67(2):902-12 PMID: 8380472
  152. Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase.
    Cell. 1993 Aug 13;74(3):463-74 PMID: 8348613
  153. Cell cycle inhibitory effects of HIV and SIV Vpr and Vpx in the yeast Schizosaccharomyces pombe.
    Virology. 1997 Mar 31;230(1):103-12 PMID: 9126266
  154. Uracil DNA glycosylase specifically interacts with Vpr of both human immunodeficiency virus type 1 and simian immunodeficiency virus of sooty mangabeys, but binding does not correlate with cell cycle arrest.
    J Virol. 1997 Jun;71(6):4842-6 PMID: 9151883
  155. Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition.
    J Virol. 1997 Jul;71(7):5382-90 PMID: 9188609
  156. Human immunodeficiency virus type 1 Vpr induces apoptosis following cell cycle arrest.
    J Virol. 1997 Jul;71(7):5579-92 PMID: 9188632
  157. Nuclear import, virion incorporation, and cell cycle arrest/differentiation are mediated by distinct functional domains of human immunodeficiency virus type 1 Vpr.
    J Virol. 1997 Sep;71(9):6339-47 PMID: 9261351
  158. Functional RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein.
    EMBO J. 1997 Aug 15;16(16):5113-22 PMID: 9305652
  159. HIV-1 Vpr suppresses immune activation and apoptosis through regulation of nuclear factor kappa B.
    Nat Med. 1997 Oct;3(10):1117-23 PMID: 9334723
  160. Human immunodeficiency virus type 1 Vpr interacts with HHR23A, a cellular protein implicated in nucleotide excision DNA repair.
    J Virol. 1997 Dec;71(12):9732-42 PMID: 9371639
  161. The zinc fingers of HIV nucleocapsid protein NCp7 direct interactions with the viral regulatory protein Vpr.
    J Biol Chem. 1997 Dec 5;272(49):30753-9 PMID: 9388214
  162. HIV-1 Vpr increases viral expression by manipulation of the cell cycle: a mechanism for selection of Vpr in vivo.
    Nat Med. 1998 Jan;4(1):65-71 PMID: 9427608
  163. Checkpoint pathways come of age.
    Cell. 1997 Dec 26;91(7):865-7 PMID: 9428508
  164. HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection.
    Genes Dev. 1998 Jan 15;12(2):175-85 PMID: 9436978
  165. The HIV-1 vpr protein acts as a negative regulator of apoptosis in a human lymphoblastoid T cell line: possible implications for the pathogenesis of AIDS.
    J Exp Med. 1998 Feb 2;187(3):403-13 PMID: 9449720
  166. Viral protein R regulates nuclear import of the HIV-1 pre-integration complex.
    EMBO J. 1998 Feb 16;17(4):909-17 PMID: 9463369
  167. Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) interacts with Lys-tRNA synthetase: implications for priming of HIV-1 reverse transcription.
    J Virol. 1998 Apr;72(4):3037-44 PMID: 9525626
  168. HIV-1 Vpr interacts with a human 34-kDa mov34 homologue, a cellular factor linked to the G2/M phase transition of the mammalian cell cycle.
    Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3419-24 PMID: 9520381
  169. Arginine residues in the C-terminus of HIV-1 Vpr are important for nuclear localization and cell cycle arrest.
    Virology. 1998 Mar 15;242(2):414-24 PMID: 9514978
  170. Human immunodeficiency virus type 1 Vpr is a positive regulator of viral transcription and infectivity in primary human macrophages.
    J Exp Med. 1998 Apr 6;187(7):1103-11 PMID: 9529326
  171. HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr.
    Mol Med. 1998 Mar;4(3):138-43 PMID: 9562972
  172. HIV transcriptional activation by the accessory protein, VPR, is mediated by the p300 co-activator.
    Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5281-6 PMID: 9560267
  173. Efficient HIV-1 replication can occur in the absence of the viral matrix protein.
    EMBO J. 1998 May 1;17(9):2699-708 PMID: 9564051
  174. Human immunodeficiency virus type 1 Vpr localization: nuclear transport of a viral protein modulated by a putative amphipathic helical structure and its relevance to biological activity.
    J Mol Biol. 1998 Apr 24;278(1):13-30 PMID: 9571031
  175. Vpr stimulates viral expression and induces cell killing in human immunodeficiency virus type 1-infected dividing Jurkat T cells.
    J Virol. 1998 Jun;72(6):4686-93 PMID: 9573232
  176. Viral protein R regulates docking of the HIV-1 preintegration complex to the nuclear pore complex.
    J Biol Chem. 1998 May 22;273(21):13347-52 PMID: 9582382
  177. Direct binding to nucleic acids by Vpr of human immunodeficiency virus type 1.
    Gene. 1998 Jun 8;212(2):157-66 PMID: 9611258
  178. Interaction of the human immunodeficiency virus type 1 Vpr protein with the nuclear pore complex.
    J Virol. 1998 Jul;72(7):6004-13 PMID: 9621063
  179. Genetic studies with the fission yeast Schizosaccharomyces pombe suggest involvement of wee1, ppa2, and rad24 in induction of cell cycle arrest by human immunodeficiency virus type 1 Vpr.
    J Virol. 2000 Mar;74(6):2636-46 PMID: 10684278
  180. Human immunodeficiency virus type 1 vpr induces apoptosis through caspase activation.
    J Virol. 2000 Apr;74(7):3105-11 PMID: 10708425
  181. Activation of transcription factors NF-kappaB and NF-IL-6 by human immunodeficiency virus type 1 protein R (Vpr) induces interleukin-8 expression.
    J Virol. 2000 May;74(10):4658-65 PMID: 10775602
Article Info
Journal
Retrovirology
Abbr.
Retrovirology
ISSN
1742-4690
DOI
10.1186/1742-4690-2-11
Email
lerouzic@cochin.inserm.fr
Published
2005-02-22
Epub
2005-00-22
Pages
11
Language
English
Region
England
NLM ID
101216893
PMCID
PMC554975
Subset
IM
External Links
PubMed source DOI: 10.1186/1742-4690-2-11 PMC Full Text View journal details
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