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PMID: 8411357 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Human immunodeficiency virus type 1 viral protein R localization in infected cells and virions.

Journal of virology ·Vol. 67 ·No. 11 ·1993-11-00 ·Pages 6542-50

Lu YL, Spearman P, Ratner L

Abstract

The subcellular localization of human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) was examined by subcellular fractionation. In HIV-1-infected peripheral blood mononuclear cells, Vpr was found in the nuclear and membrane fractions as well as the conditioned medium. Expression of Vpr without other HIV-1 proteins, in two different eukaryotic expression systems, demonstrated a predominant localization of Vpr in the nuclear matrix and chromatin extract fractions. Deletion of the carboxyl-terminal 19-amino-acid arginine-rich sequence impaired Vpr nuclear localization. Indirect immunofluorescence confirmed the nuclear localization of Vpr and also indicated a perinuclear location. Expression of Vpr alone did not result in export of the protein from the cell, but when coexpressed with the Gag protein, Vpr was exported and found in virus-like particles. A truncated Gag protein, missing the p6 sequence and a portion of the p9 sequence, was incapable of exporting Vpr from the cell. Regulation of Vpr localization may be important in the influence of this protein on virus replication.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Compartmentation Cell Nucleus/metabolism Cells, Cultured Chlorocebus aethiops DNA Primers Fluorescent Antibody Technique Gene Products, vpr/metabolism HIV-1/metabolism In Vitro Techniques Molecular Sequence Data Nuclear Proteins/metabolism Recombinant Proteins/metabolism Virion/metabolism vpr Gene Products, Human Immunodeficiency Virus
Chemicals
DNA Primers Gene Products, vpr Nuclear Proteins Recombinant Proteins vpr Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lu Y L
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Spearman P
Ratner L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.11.6542-6550.1993
Published
1993-11-00
Pages
6542-50
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238091
Subset
IM
Grants
NIAID NIH HHS · 5T32AI07172 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.11.6542-6550.1993 PMC Full Text View journal details
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