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PMID: 3872410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

V-myc- and c-myc-encoded proteins are associated with the nuclear matrix.

Molecular and cellular biology ·Vol. 5 ·No. 1 ·1985-01-00 ·Pages 114-26

Eisenman RN, Tachibana CY, Abrams HD, Hann SR

Abstract

A series of extraction procedures were applied to avian nuclei which allowed us to define three types of association of v-myc- and c-myc-encoded proteins with nuclei: (i) a major fraction (60 to 90%) which is retained in DNA- and RNA-depleted nuclei after low- and high-salt extraction, (ii) a small fraction (1%) released during nuclease digestion of DNA in intact nuclei in the presence of low-salt buffer, and (iii) a fraction of myc protein (less than 10%) extractable with salt or detergents and found to have affinity for both single- and double-stranded DNA. Immunofluorescence analysis with anti-myc peptide sera on cells extracted sequentially with nucleases and salts confirmed the idea that myc proteins were associated with a complex residual nuclear structure (matrix-lamin fraction) which also contained avian nuclear lamin protein. Dispersal of myc proteins into the cytoplasm was found to occur during mitosis. Both c-myc and v-myc proteins were associated with the matrix-lamin, suggesting that the function of myc may relate to nuclear structural organization.

MeSH Terms
Animals Cell Nucleus/analysis,ultrastructure Cell Transformation, Viral DNA-Binding Proteins/analysis Deoxyribonucleases Gene Products, gag Lamins Molecular Weight Nucleoproteins/metabolism Oncogenes Osmolar Concentration Quail Ribonucleases Viral Proteins/metabolism
Chemicals
DNA-Binding Proteins Gene Products, gag Lamins Nucleoproteins Viral Proteins Deoxyribonucleases Ribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Eisenman R N
Tachibana C Y
Abrams H D
Hann S R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.1.114-126.1985
Published
1985-01-00
Pages
114-26
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366685
Subset
IM
Grants
NCI NIH HHS · CA 20525 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.1.114-126.1985 PMC Full Text View journal details
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