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PMID: 9621063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction of the human immunodeficiency virus type 1 Vpr protein with the nuclear pore complex.

Journal of virology ·Vol. 72 ·No. 7 ·1998-07-00 ·Pages 6004-13

Fouchier RA, Meyer BE, Simon JH, Fischer U, Albright AV, González-Scarano F, Malim MH

Abstract

The Vpr protein of human immunodeficiency virus type 1 (HIV-1) performs a number of functions that are associated with the nucleus. Vpr enhances the nuclear import of postentry viral nucleoprotein complexes, arrests proliferating cells in the G2 phase of the cell cycle, and acts as a modest transcriptional activator. For this paper, we have investigated the nuclear import of Vpr. Although Vpr does not encode a sequence that is recognizable as a nuclear localization signal (NLS), Vpr functions as a transferable NLS both in somatic cells and in Xenopus laevis oocytes. In certain contexts, Vpr also mediates substantial accumulation at the nuclear envelope and, in particular, at nuclear pore complexes (NPCs). Consistent with this, Vpr is shown to interact specifically with nucleoporin phenylalanine-glycine (FG)-repeat regions. These findings not only demonstrate that Vpr harbors a bona fide NLS but also raise the possibility that one (or more) of Vpr's functions may take place at the NPC.

MeSH Terms
Biological Transport Cells, Cultured Gene Products, vpr/metabolism HIV-1/chemistry,physiology HeLa Cells Humans Macrophages/virology Nuclear Envelope/metabolism Nucleoproteins/metabolism Virus Replication vpr Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, vpr Nucleoproteins vpr Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fouchier R A
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6148, USA.
Meyer B E
Simon J H
Fischer U
Albright A V
González-Scarano F
Malim M H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.7.6004-6013.1998
Published
1998-07-00
Pages
6004-13
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC110405
Subset
IM
Grants
NIAID NIH HHS · AI41933 · United States
External Links
PubMed source DOI: 10.1128/JVI.72.7.6004-6013.1998 PMC Full Text View journal details
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