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PMID: 10775602 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activation of transcription factors NF-kappaB and NF-IL-6 by human immunodeficiency virus type 1 protein R (Vpr) induces interleukin-8 expression.

Journal of virology ·Vol. 74 ·No. 10 ·2000-05-00 ·Pages 4658-65

Roux P, Alfieri C, Hrimech M, Cohen EA, Tanner JE

Abstract

Human immunodeficiency virus (HIV)-positive individuals express elevated levels of interleukin-8 (IL-8), which is believed to be responsible for some of the clinical manifestations occurring during AIDS. We report here that virion-derived HIV type 1 (HIV-1) protein R (Vpr) increased IL-8 expression in primary T cells and macrophages, as well as in the T-cell line Jurkat, the monocytic cell line U937, and the epithelial cell line A549. Vpr appeared to increase IL-8 expression and IL-8 promoter activity by activating transcription factors NF-kappaB and NF-IL-6. Elevated Vpr was also shown to increase transcription of the NF-kappaB and NF-IL-6 enhancer-containing viral promoters for HIV, cytomegalovirus, and simian virus 40, as well as increase the expression of IL-6 and IL-10 in primary macrophages and in A549 cells, tumor necrosis factor alpha expression in primary T cells, and IL-6 and gamma interferon expression in U937 cells. These results suggest a new role for Vpr in the pathogenesis of HIV infection, namely, the activation of transcription factors NF-IL-6 and NF-kappaB.

MeSH Terms
CCAAT-Enhancer-Binding Proteins Cell Line Cells, Cultured Cytokines/biosynthesis DNA-Binding Proteins/metabolism Gene Products, vpr/genetics,metabolism HIV-1/genetics,metabolism Humans Interleukin-8/biosynthesis,genetics Jurkat Cells Macrophages/metabolism NF-kappa B/metabolism Nuclear Proteins/metabolism Plasmids Promoter Regions, Genetic T-Lymphocytes/metabolism Transcription Factors/metabolism vpr Gene Products, Human Immunodeficiency Virus
Chemicals
CCAAT-Enhancer-Binding Proteins Cytokines DNA-Binding Proteins Gene Products, vpr Interleukin-8 NF-kappa B Nuclear Proteins Transcription Factors vpr Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Roux P
Laboratory of Molecular Virology, Ste-Justine Hospital Research Center, Canada.
Alfieri C
Hrimech M
Cohen E A
Tanner J E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.74.10.4658-4665.2000
Published
2000-05-00
Pages
4658-65
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC111986
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.74.10.4658-4665.2000 PMC Full Text View journal details
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