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PMID: 12510154 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Upregulation of survivin by HIV-1 Vpr.

Apoptosis : an international journal on programmed cell death ·Vol. 8 ·No. 1 ·2003-01-00 ·Pages 71-9

Zhu Y, Roshal M, Li F, Blackett J, Planelles V

Abstract

The human survivin gene belongs to the family of inhibitor of apoptosis proteins (IAP) and is involved in apoptosis inhibition and regulation of cell division. The survivin gene is the only member of the IAP family whose expression is known to be regulated through the cell cycle. Survivin expression reaches the highest levels during the G(2)/M transition and then is rapidly degraded during the G(1) phase. Here we report that the human immunodeficiency virus type 1 (HIV-1) upregulates Survivin expression via survivin promoter transactivation. Vpr, an HIV-1 accessory protein that induces cell cycle arrest in G(2)/M, is necessary and sufficient for this effect. Blocking Vpr-induced G(2)/M arrest leads to elimination of the survivin promoter transactivation by Vpr. Our results suggest that Survivin may be actively involved in regulating cell viability during HIV-1 infection.

MeSH Terms

Blotting, Western Caffeine/pharmacology Caspase 3 Caspases/metabolism Cell Cycle Cell Line Cell Survival G1 Phase G2 Phase Gene Products, vpr/metabolism Genes, Reporter Genetic Vectors/genetics Green Fluorescent Proteins HIV Infections HIV-1/metabolism HeLa Cells Humans Inhibitor of Apoptosis Proteins Luciferases/metabolism Luminescent Proteins/metabolism Microscopy, Fluorescence Microtubule-Associated Proteins/biosynthesis Models, Genetic Neoplasm Proteins Plasmids/metabolism Promoter Regions, Genetic Survivin Time Factors Transcriptional Activation Transfection Up-Regulation beta-Galactosidase/metabolism vpr Gene Products, Human Immunodeficiency Virus

Chemicals

BIRC5 protein, human Gene Products, vpr Inhibitor of Apoptosis Proteins Luminescent Proteins Microtubule-Associated Proteins Neoplasm Proteins Survivin vpr Gene Products, Human Immunodeficiency Virus Green Fluorescent Proteins Caffeine Luciferases beta-Galactosidase CASP3 protein, human Caspase 3 Caspases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Zhu Y

Department of Microbiology and Immunology, University of Rochester, Rochester, New York 14642, USA.

Roshal M

Li F

Blackett J

Planelles V

Article Info

Journal

Apoptosis : an international journal on programmed cell death

Abbr.

Apoptosis

ISSN

1360-8185

DOI

10.1023/a:1021653119934

Published

2003-01-00

Pages

71-9

Language

English

Region

Netherlands

NLM ID

9712129

Subset

Grants

NIAID NIH HHS · R01AI49057 · United States

NIAID NIH HHS · R29AI41407 · United States

External Links

PubMed source DOI: 10.1023/a:1021653119934 View journal details

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Upregulation of survivin by HIV-1 Vpr.

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