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PMID: 15467781 Published · ppublish English Journal Article

Localization of sequences in wheat endosperm protein genes which confer tissue-specific expression in tobacco.

The EMBO journal ·Vol. 6 ·No. 12 ·1987-00-00 ·Pages 3559-64

Colot V, Robert LS, Kavanagh TA, Bevan MW, Thompson RD

Abstract

The developing cereal grain accumulates large quantities of proteins which are unique to the endosperm tissue. The DNA sequences which determine their endosperm-specific expression have not yet been identified. In the absence of a suitable transformation-regeneration system for cereals, we have investigated whether chimaeric genes consisting of low mol. wt (LMW) and high mol. wt (HMW) glutenin gene upstream sequences coupled to the coding region of the bacterial chloramphenicol acetyl transferase (CAT) gene could be specifically expressed in transgenic tobacco. The fusions, made in a Ti-derived binary vector, were introduced into tobacco via Agrobacterium tumefaciens-mediated transformation and their activity assayed. Both the LMW and HMW glutenin chimaeric genes exhibited endosperm-specific CAT activity in the transformed plants. In addition, a deletion series of the LMW glutenin sequence indicated that sequences present between 326 bp and 160 bp upstream of the transcription start point are necessary to confer endosperm-specific CAT activity.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Colot V
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ, UK.
Robert L S
Kavanagh T A
Bevan M W
Thompson R D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-00-00
Pages
3559-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553821
External Links
PubMed source PMC Full Text View journal details
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