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PMID: 15981330 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Binding of a nuclear factor to a consensus sequence in the 5' flanking region of zein genes from maize.

The EMBO journal ·Vol. 6 ·No. 1 ·1987-01-00 ·Pages 17-22

Maier UG, Brown JW, Tologcyzki C, Feix G

Abstract

The genomic organization of the zein structural genes and of regulatory loci influencing their expression suggests that control of zein gene expression will involve interactions between cis elements in the flanking DNA sequences and products from trans-acting genes. The interaction between fragments from the 5' flanking region of a zein gene and specific, double-stranded oligonucleotides with crude nuclear extracts from maize endosperm have been studied by nitrocellulose filter binding, gel retention and DNase I footprinting assays. Specific binding of a nuclear factor was observed and the exact position of the protein binding site was determined. The 22-nt binding site included 14 bp of a 15-bp sequence conserved in all zein genes.

MeSH Terms
Consensus Sequence/genetics DNA Footprinting Genes, Plant/genetics Nuclear Proteins/genetics Plant Proteins/genetics Protein Binding/genetics Zea mays/genetics Zein/genetics
Chemicals
Nuclear Proteins Plant Proteins Zein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Maier U G
Institute for Biology II, Albert Ludwigs Universität, D-7800 Freiburg, FRG.
Brown J W
Tologcyzki C
Feix G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-01-00
Pages
17-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553350
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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