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PMID: 15340082 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Swi/Snf chromatin remodeling complex is required for ribosomal DNA and telomeric silencing in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 24 ·No. 18 ·2004-09-00 ·Pages 8227-35

Dror V, Winston F

Abstract

The Swi/Snf chromatin remodeling complex has been previously demonstrated to be required for transcriptional activation and repression of a subset of genes in Saccharomyces cerevisiae. In this work we demonstrate that Swi/Snf is also required for repression of RNA polymerase II-dependent transcription in the ribosomal DNA (rDNA) locus (rDNA silencing). This repression appears to be independent of both Sir2 and Set1, two factors known to be required for rDNA silencing. In contrast to many other rDNA silencing mutants that have elevated levels of rDNA recombination, snf2Delta mutants have a significantly decreased level of rDNA recombination. Additional studies have demonstrated that Swi/Snf is also required for silencing of genes near telomeres while having no detectable effect on silencing of HML or HMR.

MeSH Terms
Adenosine Triphosphatases Base Sequence Chromatin Assembly and Disassembly DNA, Fungal/genetics DNA, Ribosomal/genetics DNA-Binding Proteins/genetics,metabolism Gene Silencing Genes, Fungal Histone Deacetylases/genetics,metabolism Histone-Lysine N-Methyltransferase Mutagenesis Nuclear Proteins/genetics,metabolism Promoter Regions, Genetic Recombination, Genetic Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Silent Information Regulator Proteins, Saccharomyces cerevisiae/genetics,metabolism Sirtuin 2 Sirtuins/genetics,metabolism Telomere/genetics,metabolism Trans-Activators/genetics,metabolism Transcription Factors/genetics,metabolism beta-Fructofuranosidase/genetics
Chemicals
DNA, Fungal DNA, Ribosomal DNA-Binding Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins Silent Information Regulator Proteins, Saccharomyces cerevisiae Trans-Activators Transcription Factors Histone-Lysine N-Methyltransferase SET1 protein, S cerevisiae SUC2 protein, S cerevisiae beta-Fructofuranosidase SIR2 protein, S cerevisiae Sirtuin 2 Sirtuins Histone Deacetylases Adenosine Triphosphatases SNF2 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dror Vardit
Department of Genetics, Harvard Medical School, 77 Ave. Louis Pasteur, Boston, MA 02115, USA.
Winston Fred
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.18.8227-8235.2004
Published
2004-09-00
Pages
8227-35
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC515061
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032967 · United States
NIGMS NIH HHS · R37 GM032967 · United States
NIGMS NIH HHS · GM32967 · United States
External Links
PubMed source DOI: 10.1128/MCB.24.18.8227-8235.2004 PMC Full Text View journal details
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