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PMID: 10388812 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A general requirement for the Sin3-Rpd3 histone deacetylase complex in regulating silencing in Saccharomyces cerevisiae.

Genetics ·Vol. 152 ·No. 3 ·1999-07-00 ·Pages 921-32

Sun ZW, Hampsey M

Abstract

The Sin3-Rpd3 histone deacetylase complex, conserved between human and yeast, represses transcription when targeted by promoter-specific transcription factors. SIN3 and RPD3 also affect transcriptional silencing at the HM mating loci and at telomeres in yeast. Interestingly, however, deletion of the SIN3 and RPD3 genes enhances silencing, implying that the Sin3-Rpd3 complex functions to counteract, rather than to establish or maintain, silencing. Here we demonstrate that Sin3, Rpd3, and Sap30, a novel component of the Sin3-Rpd3 complex, affect silencing not only at the HMR and telomeric loci, but also at the rDNA locus. The effects on silencing at all three loci are dependent upon the histone deacetylase activity of Rpd3. Enhanced silencing associated with sin3Delta, rpd3Delta, and sap30Delta is differentially dependent upon Sir2 and Sir4 at the telomeric and rDNA loci and is also dependent upon the ubiquitin-conjugating enzyme Rad6 (Ubc2). We also show that the Cac3 subunit of the CAF-I chromatin assembly factor and Sin3-Rpd3 exert antagonistic effects on silencing. Strikingly, deletion of GCN5, which encodes a histone acetyltransferase, enhances silencing in a manner similar to deletion of RPD3. A model that integrates the effects of rpd3Delta, gcn5Delta, and cac3Delta on silencing is proposed.

MeSH Terms
Acetylation DNA-Binding Proteins Fungal Proteins/genetics Genotype Histone Acetyltransferases Histone Deacetylases/metabolism Histones/physiology Ligases/genetics Models, Biological Mutagenesis Protein Kinases/genetics Repressor Proteins Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Transcription Factors/genetics,physiology Ubiquitin-Conjugating Enzymes
Chemicals
DNA-Binding Proteins Fungal Proteins Histones Repressor Proteins SIN3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors GCN5 protein, S cerevisiae Histone Acetyltransferases RAD6 protein, S cerevisiae Ubiquitin-Conjugating Enzymes Protein Kinases RPD3 protein, S cerevisiae Histone Deacetylases Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sun Z W
Department of Biochemistry, Division of Nucleic Acids Enzymology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
Hampsey M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/152.3.921
Published
1999-07-00
Pages
921-32
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460667
Subset
IM
Grants
NIGMS NIH HHS · GM-39484 · United States
External Links
PubMed source DOI: 10.1093/genetics/152.3.921 PMC Full Text View journal details
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