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PMID: 8816485 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The dose of a putative ubiquitin-specific protease affects position-effect variegation in Drosophila melanogaster.

Molecular and cellular biology ·Vol. 16 ·No. 10 ·1996-10-00 ·Pages 5717-25

Henchoz S, De Rubertis F, Pauli D, Spierer P

Abstract

A dominant insertional P-element mutation enhances position-effect variegation in Drosophila melanogaster. The mutation is homozygous, viable, and fertile and maps at 64E on the third chromosome. The corresponding gene was cloned by transposon tagging. Insertion of the transposon upstream of the open reading frame correlates with a strong reduction of transcript level. A transgene was constructed with the cDNA and found to have the effect opposite from that of the mutation, namely, to suppress variegation. Sequencing of the cDNA reveals a large open reading frame encoding a putative ubiquitin-specific protease (Ubp). Ubiquitin marks various proteins, frequently for proteasome-dependent degradation. Ubps can cleave the ubiquitin part from these proteins. We discuss the link established here between a deubiquitinating enzyme and epigenetic silencing processes.

MeSH Terms
Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence Chromosome Mapping Crosses, Genetic Drosophila melanogaster/enzymology,genetics Embryo, Nonmammalian Endopeptidases/genetics,metabolism Female Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Genes, Homeobox Genes, Insect Homozygote Male Molecular Sequence Data Mutagenesis, Insertional Open Reading Frames Pigmentation Recombination, Genetic Restriction Mapping Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Endopeptidases ubiquitin-Nalpha-protein hydrolase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Henchoz S
Department of Zoology and Animal Biology, University of Geneva, Switzerland.
De Rubertis F
Pauli D
Spierer P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.10.5717
Published
1996-10-00
Pages
5717-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231572
Subset
IM
Databases
GENBANK
X99211
External Links
PubMed source DOI: 10.1128/MCB.16.10.5717 PMC Full Text View journal details
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