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PMID: 8668146 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

TFG/TAF30/ANC1, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9.

Molecular and cellular biology ·Vol. 16 ·No. 7 ·1996-07-00 ·Pages 3308-16

Cairns BR, Henry NL, Kornberg RD

Abstract

The SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products are all required for proper transcriptional control of many genes in the yeast Saccharomyces cerevisiae. Genetic studies indicated that these gene products might form a multiprotein SWI/SNF complex important for chromatin transitions preceding transcription from RNA polymerase II promoters. Biochemical studies identified a SWI/SNF complex containing these and at least six additional polypeptides. Here we show that the 29-kDa component of the SWI/SNF complex is identical to TFG3/TAF30/ANC1. Thus, a component of the SWI/SNF complex is also a member of the TFIIF and TFIID transcription complexes. TFG3 interacted with the SNF5 component of the SWI/SNF complex in protein interaction blots. TFG3 is significantly similar to ENL and AF-9, two proteins implicated in human acute leukemia. These results suggest that ENL and AF-9 proteins interact with the SNF5 component of the human SWI/SNF complex and raise the possibility that the SWI/SNF complex is involved in acute leukemia.

MeSH Terms
Amino Acid Sequence Fungal Proteins/chemistry,genetics Genes, Fungal Humans Molecular Sequence Data Neoplasm Proteins Nuclear Proteins/chemistry,genetics Recombinant Fusion Proteins/biosynthesis,chemistry,isolation & purification Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Transcription Factor TFIID Transcription Factors/chemistry,genetics,isolation & purification
Chemicals
Fungal Proteins MLLT1 protein, human MLLT3 protein, human Neoplasm Proteins Nuclear Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins TAF14 protein, S cerevisiae Transcription Factor TFIID Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cairns B R
Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.
Henry N L
Kornberg R D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.7.3308
Published
1996-07-00
Pages
3308-16
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231325
Subset
IM
Grants
NIGMS NIH HHS · GM36659 · United States
External Links
PubMed source DOI: 10.1128/MCB.16.7.3308 PMC Full Text View journal details
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