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PMID: 7908117 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification and characterization of Drosophila relatives of the yeast transcriptional activator SNF2/SWI2.

Molecular and cellular biology ·Vol. 14 ·No. 4 ·1994-04-00 ·Pages 2225-34

Elfring LK, Deuring R, McCallum CM, Peterson CL, Tamkun JW

Abstract

The Drosophila brahma (brm) gene encodes an activator of homeotic genes that is highly related to the yeast transcriptional activator SWI2 (SNF2), a potential helicase. To determine whether brm is a functional homolog of SWI2 or merely a member of a family of SWI2-related genes, we searched for additional Drosophila genes related to SWI2 and examined their function in yeast cells. In addition to brm, we identified one other Drosophila relative of SWI2: the closely related ISWI gene. The 1,027-residue ISWI protein contains the DNA-dependent ATPase domain characteristic of the SWI2 protein family but lacks the three other domains common to brm and SWI2. In contrast, the ISWI protein is highly related (70% identical) to the human hSNF2L protein over its entire length, suggesting that they may be functional homologs. The DNA-dependent ATPase domains of brm and SWI2, but not ISWI, are functionally interchangeable; a chimeric SWI2-brm protein partially rescued the slow growth of swi2- cells and supported transcriptional activation mediated by the glucocorticoid receptor in vivo in yeast cells. These findings indicate that brm is the closest Drosophila relative of SWI2 and suggest that brm and SWI2 play similar roles in transcriptional activation.

Related Genes
ISWI SNF2 SW12 brm
MeSH Terms
Adenosine Triphosphatases/genetics Amino Acid Sequence Animals Base Sequence Cell Cycle Proteins DNA Helicases DNA Primers DNA-Binding Proteins/genetics Drosophila/embryology,genetics,metabolism Drosophila Proteins Embryo, Nonmammalian/metabolism Genes, Homeobox In Situ Hybridization Molecular Sequence Data Nuclear Proteins Polymerase Chain Reaction Protein Biosynthesis RNA, Messenger/biosynthesis,metabolism Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Trans-Activators/biosynthesis,genetics Transcription Factors/biosynthesis,genetics
Chemicals
Cell Cycle Proteins DNA Primers DNA-Binding Proteins Drosophila Proteins ISWI protein Nuclear Proteins RNA, Messenger Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors brm protein, Drosophila Adenosine Triphosphatases SNF2 protein, S cerevisiae DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Elfring L K
Department of Biology, University of California, Santa Cruz 95064.
Deuring R
McCallum C M
Peterson C L
Tamkun J W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.4.2225-2234.1994
Published
1994-04-00
Pages
2225-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358589
Subset
IM
Grants
NIGMS NIH HHS · R01 GM049650 · United States
NIGMS NIH HHS · R37 GM049650 · United States
Databases
GENBANK
L27127
External Links
PubMed source DOI: 10.1128/mcb.14.4.2225-2234.1994 PMC Full Text View journal details
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