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PMID: 8223438 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor.

The EMBO journal ·Vol. 12 ·No. 11 ·1993-11-00 ·Pages 4279-90

Muchardt C, Yaniv M

Abstract

Several of the SNF and SWI genes of Saccharomyces cerevisiae code for proteins believed to assist transcriptional activators by relieving nucleosome repression. One of these proteins, SNF2/SWI2, has a homologue in Drosophila, a regulator of homeotic genes known as brahma or brm. In this report, we show that a counterpart of SNF2/SWI2 also exists in mice and humans. The human protein, designated hbrm, is a 180 kDa nuclear factor that can function as a transcriptional activator when fused to a heterologous DNA binding domain. The mouse homologue of hbrm is expressed in all mouse organs tested while hbrm was detected in some but not all investigated human cell lines. In cells failing to express the endogenous gene, transfected hbrm cooperates with the glucocorticoid receptor (GR) in transcriptional activation. However, hbrm had no effect on the activity of several other transcription factors, including the homeoprotein HNF-1. The co-operation between hbrm and GR required the DNA binding domain of GR and two separated regions of the hbrm protein, including a domain with homology to known helicases.

Related Genes
SNF2 SWI2 brm
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cells, Cultured Cloning, Molecular DNA Helicases/metabolism DNA Mutational Analysis DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation Genes, Insect Genes, Reporter Humans Mice Molecular Sequence Data Nuclear Proteins Receptors, Glucocorticoid/metabolism Recombinant Fusion Proteins Sequence Homology, Amino Acid Signal Transduction Species Specificity Transcription Factors/genetics,metabolism Transcription, Genetic Transcriptional Activation
Chemicals
DNA-Binding Proteins Nuclear Proteins Receptors, Glucocorticoid Recombinant Fusion Proteins SMARCA1 protein, human SMARCA2 protein, human Smarca2 protein, mouse Transcription Factors SMARCA4 protein, human Smarca4 protein, mouse DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Muchardt C
Unité des Virus Oncogènes, UA1644 du CNRS, Département des Biotechnologies, Institut Pasteur, Paris, France.
Yaniv M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-11-00
Pages
4279-90
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413724
Subset
IM
Databases
GENBANK
X72889
External Links
PubMed source PMC Full Text View journal details
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