Abstract
We have isolated a novel gene (NUM1) with unusual internal periodicity. The NUM1 gene encodes a 313 kDa protein with a potential Ca2+ binding site and a central domain containing 12 almost identical tandem repeats of a 64 amino acid polypeptide. num1-disrupted strains grow normally, but contain many budded cells with two nuclei in the mother cell instead of a single nucleus at the bud neck, while all unbudded cells are uninucleate. This indicates that most G2 nuclei divide in the mother before migrating to the neck, followed by the migration of one of the two daughter nuclei into the bud. Furthermore, haploid num1 strains tend to diploidize during mitosis, and homozygous num1 diploid or tetraploid cells sporulate to form many budded asci with up to eight haploid or diploid spores, respectively, indicating that meiosis starts before nuclear redistribution and cytokinesis. Our data suggest that the NUM1 protein is involved in the interaction of the G2 nucleus with the bud neck.
MeSH Terms
Amino Acid Sequence
Base Sequence
Calcium-Binding Proteins/genetics,metabolism
Cell Nucleus/physiology
Cytoskeletal Proteins
DNA, Fungal/genetics
Fungal Proteins/genetics,metabolism
Genes, Fungal
Mitosis
Molecular Sequence Data
Plasmids
RNA, Fungal/analysis
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae Proteins
Chemicals
Calcium-Binding Proteins
Cytoskeletal Proteins
DNA, Fungal
Fungal Proteins
NUM1 protein, S cerevisiae
RNA, Fungal
Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kormanec J
Max-Planck-Institut für experimentelle Medizin, Abteilung Chemie, Göttingen, FRG.
Schaaff-Gerstenschläger I
Zimmermann F K
Perecko D
Küntzel H
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