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PMID: 10205178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Continuous and widespread roles for the Swi-Snf complex in transcription.

The EMBO journal ·Vol. 18 ·No. 8 ·1999-04-15 ·Pages 2254-64

Biggar SR, Crabtree GR

Abstract

Chromatin presents a significant obstacle to transcription, but two means of overcoming its repressive effects, histone acetylation and the activities of the Swi-Snf complex, have been proposed. Histone acetylation and Swi-Snf activity have been shown to be crucial for transcriptional induction and to facilitate binding of transcription factors to DNA. By regulating the activity of the Swi-Snf complex in vivo, we found that active transcription requires continuous Swi-Snf function, demonstrating a role for this complex beyond the induction of transcription. Despite the presumably generalized packaging of genes into chromatin, previous studies have indicated that the transcriptional requirements for the histone acetyltransferase, Gcn5, and the Swi-Snf complex are limited to a handful of genes. However, inactivating Swi-Snf function in cells also lacking GCN5 revealed defects in transcription of several genes previously thought to be SWI-SNF- and GCN5-independent. These findings suggest that chromatin remodeling plays a widespread role in gene expression and that these two chromatin remodeling activities perform independent and overlapping functions during transcriptional activation.

MeSH Terms
DNA-Binding Proteins/physiology Fungal Proteins/physiology Histone Acetyltransferases Mutation Nuclear Proteins Protein Kinases/physiology Saccharomyces cerevisiae Proteins Transcription Factors/physiology Transcription, Genetic/physiology
Chemicals
DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Nuclear Proteins Saccharomyces cerevisiae Proteins Transcription Factors GCN5 protein, S cerevisiae Histone Acetyltransferases Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Biggar S R
Departments of Developmental Biology and Pathology, Stanford University Medical School, Stanford, CA 94305, USA.
Crabtree G R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.8.2254
Published
1999-04-15
Pages
2254-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171308
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/18.8.2254 PMC Full Text View journal details
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