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PMID: 7813441 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential repression of transcription factor binding by histone H1 is regulated by the core histone amino termini.

The EMBO journal ·Vol. 13 ·No. 24 ·1994-12-15 ·Pages 6031-40

Juan LJ, Utley RT, Adams CC, Vettese-Dadey M, Workman JL

Abstract

In order to investigate the interrelated roles of nucleosome cores and histone H1 in transcription repression, we have employed a purified system to analyze the function of H1 in the repression of transcription factor binding to nucleosomes. H1 binding to nucleosome cores resulted in the repression of USF binding to nucleosomes. By contrast, H1 only slightly inhibited the binding of GAL4-AH, indicating that H1 differentially represses the binding of factors with different DNA-binding domains. H1-mediated repression of factor binding was dependent on the core histone amino-terminal tails. Removal of these domains alleviated H1-mediated repression and increased acetylation of these domains partly alleviated repression by H1. H1 binding assays suggest a less stable interaction of histone H1 with the core particle in the absence of the amino termini.

MeSH Terms
Acetylation Base Sequence DNA-Binding Proteins/metabolism Fungal Proteins/metabolism HeLa Cells Histones/pharmacology Humans Molecular Sequence Data Nucleosomes/metabolism Protein Binding/drug effects Saccharomyces cerevisiae Proteins Structure-Activity Relationship Transcription Factors/metabolism Upstream Stimulatory Factors
Chemicals
DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Histones Nucleosomes Saccharomyces cerevisiae Proteins Transcription Factors USF1 protein, human Upstream Stimulatory Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Juan L J
Center for Gene Regulation, Pennsylvania State University, University Park 16802-4500.
Utley R T
Adams C C
Vettese-Dadey M
Workman J L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-12-15
Pages
6031-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395580
Subset
IM
Grants
NIGMS NIH HHS · R01-GM47867 · United States
External Links
PubMed source PMC Full Text View journal details
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