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PMID: 1996118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Formaldehyde cross-linking and immunoprecipitation demonstrate developmental changes in H1 association with transcriptionally active genes.

Molecular and cellular biology ·Vol. 11 ·No. 3 ·1991-03-00 ·Pages 1729-33

Dedon PC, Soults JA, Allis CD, Gorovsky MA

Abstract

The in vivo association of histone H1 with specific genes in Tetrahymena thermophila was studied by using a simplified cross-linking and immunoprecipitation technique. Four genes were analyzed whose activities vary in three different developmental states (logarithmic growth, starvation, and conjugation). Hybridization of the immunoprecipitated DNA to cloned probes showed an inverse correlation between the level of immunoprecipitation with H1 antiserum and transcriptional activity. This represents the first demonstration of an alteration in histone H1-DNA interaction associated with developmental changes in transcriptional activity.

Related Genes
cnjB ngoA
MeSH Terms
Actins/genetics Animals Conjugation, Genetic Cross-Linking Reagents DNA, Ribosomal/genetics DNA-Binding Proteins/metabolism Formaldehyde Gene Expression Histones/metabolism Nucleosomes/ultrastructure Precipitin Tests Tetrahymena/genetics Transcription, Genetic
Chemicals
Actins Cross-Linking Reagents DNA, Ribosomal DNA-Binding Proteins Histones Nucleosomes Formaldehyde
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dedon P C
Department of Biology, University of Rochester, New York 14627.
Soults J A
Allis C D
Gorovsky M A
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34 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.3.1729-1733.1991
Published
1991-03-00
Pages
1729-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369483
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.3.1729-1733.1991 PMC Full Text View journal details
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