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PMID: 3464976 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An intervening sequence in an unusual histone H1 gene of Tetrahymena thermophila.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 22 ·1986-11-00 ·Pages 8674-8

Wu M, Allis CD, Richman R, Cook RG, Gorovsky MA

Abstract

An intervening sequence of 254 base pairs interrupts the coding region of the single gene for macronuclear histone H1 of the ciliated protozoan, Tetrahymena thermophila. The intervening sequence has splice junctions similar to those found in RNA polymerase II genes of other organisms. No obvious similarities are observed between this intron and the self-splicing intervening sequence of the Tetrahymena ribosomal gene. The derived amino acid sequence describes a small extremely basic H1 protein missing most of the central hydrophobic domain that is conserved in all other H1 proteins. Macronuclei divide amitotically, without chromosome condensation, suggesting the conserved globular domain of H1 plays a role in higher-order chromatin structure.

MeSH Terms
Amino Acid Sequence Histones/analysis,genetics Introns Tetrahymena/genetics
Chemicals
Histones
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wu M
Allis C D
Richman R
Cook R G
Gorovsky M A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.22.8674
Published
1986-11-00
Pages
8674-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386993
Subset
IM
Databases
GENBANK
M14854
External Links
PubMed source DOI: 10.1073/pnas.83.22.8674 PMC Full Text View journal details
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