Home LiteratureArticle Details
PMID: 2017359 Published · ppublish English Journal Article

Distribution of high mobility group proteins 1/2, E and 14/17 and linker histones H1 and H5 on transcribed and non-transcribed regions of chicken erythrocyte chromatin.

Nucleic acids research ·Vol. 19 ·No. 4 ·1991-02-25 ·Pages 717-25

Postnikov YV, Shick VV, Belyavsky AV, Khrapko KR, Brodolin KL, Nikolskaya TA, Mirzabekov AD

Abstract

Quantitative analysis of distribution of chromosomal proteins on single copy DNA sequences has been further developed. Our approach consists of DNA-protein crosslinking within whole cells or isolated nuclei, specific immunoaffinity isolation of crosslinked complexes via protein and identification of crosslinked DNA by hybridisation with single-stranded DNA probes. The present study shows that transcribed chromatin of chicken embryonic erythrocyte beta globin gene is characterized by about 1.5-2.5-fold higher density of HMG 14/17 and 2-fold lower density of H1 and H5 as compared with non-transcribed chromatin of ovalbumin and lysozyme genes, whereas HMG 1/2, E proteins were equally distributed between DNA of both transcribed and non-transcribed genes. The depletion of H1/H5 in beta globin sequences was verified by the 'protein image' hybridisation technique (1). The DNase I hypersensitive site located 5' upstream from beta globin gene is deficient in all the proteins assayed, what implies a drastic disruption in the nucleosomal array. Minor quantitative changes of protein pattern suggest transient local perturbation of the chromatin on transcription.

MeSH Terms
Animals Blotting, Western Chickens Chromatin Cross-Linking Reagents DNA Probes Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Erythrocytes/metabolism High Mobility Group Proteins/genetics Histones/genetics Muramidase/genetics Nucleic Acid Hybridization Ovalbumin/genetics Precipitin Tests Restriction Mapping Transcription, Genetic
Chemicals
Chromatin Cross-Linking Reagents DNA Probes High Mobility Group Proteins Histones Ovalbumin Muramidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Postnikov Y V
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Shick V V
Belyavsky A V
Khrapko K R
Brodolin K L
Nikolskaya T A
Mirzabekov A D
References (55)
55 references, click to expand
  1. Induction by torsional stress of an altered DNA conformation 5' upstream of the gene for a high mobility group protein from trout and specific binding to flanking sequences by the gene product HMG-T.
    Biochemistry. 1988 Jan 26;27(2):576-81 PMID: 2831970
  2. Nonhistone protein HMG1 removes the transcriptional block caused by left-handed Z-form segment in a supercoiled DNA.
    Biochem Biophys Res Commun. 1988 May 31;153(1):334-9 PMID: 3377790
  3. Mapping protein-DNA interactions in vivo with formaldehyde: evidence that histone H4 is retained on a highly transcribed gene.
    Cell. 1988 Jun 17;53(6):937-47 PMID: 2454748
  4. Erythroid-specific gene chromatin has an altered association with linker histones.
    Nucleic Acids Res. 1988 Jul 11;16(13):5915-26 PMID: 3399383
  5. Specific recognition of cruciform DNA by nuclear protein HMG1.
    Science. 1989 Feb 24;243(4894 Pt 1):1056-9 PMID: 2922595
  6. Change in the pattern of histone binding to DNA upon transcriptional activation.
    Cell. 1989 Jul 14;58(1):27-36 PMID: 2502314
  7. Mapping DNA-protein interactions by cross-linking.
    Methods Enzymol. 1989;170:386-408 PMID: 2770546
  8. High mobility group protein 1 preferentially conserves torsion in negatively supercoiled DNA.
    Biochemistry. 1989 Jun 27;28(13):5658-64 PMID: 2550058
  9. High mobility group proteins 1 and 2 function as general class II transcription factors.
    Biochemistry. 1990 Jul 3;29(26):6295-302 PMID: 2119799
  10. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.
    Biochem J. 1973 Jul;133(3):529-39 PMID: 4733239
  11. The ovalbumin gene: cloning of the natural gene.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3688-92 PMID: 358194
  12. Exchange of histone H1 between segments of chromatin.
    J Mol Biol. 1981 Mar 15;146(4):513-37 PMID: 7277492
  13. A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion.
    Cell. 1981 Nov;27(1 Pt 2):45-55 PMID: 6276024
  14. Nucleosomes containing histones H1 or H5 are closely interspersed in chromatin.
    Nucleic Acids Res. 1982 Apr 10;10(7):2323-35 PMID: 6178082
  15. The chicken beta globin gene region. Delineation of transcription units and developmental regulation of interspersed DNA repeats.
    J Biol Chem. 1982 Sep 25;257(18):11015-23 PMID: 6286679
  16. The nuclease sensitivity of active genes.
    Nucleic Acids Res. 1983 Feb 11;11(3):753-72 PMID: 6300766
  17. Enzyme-linked immunosorbent assay for screening monoclonal antibody production using enzyme-labeled second antibody.
    Methods Enzymol. 1983;92:168-74 PMID: 6343762
  18. Iodine monochloride (IC1) iodination techniques.
    Methods Enzymol. 1983;92:277-92 PMID: 6855615
  19. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  20. High mobility group proteins: abundance, turnover, and relationship to transcriptionally active chromatin.
    Biochemistry. 1983 Oct 11;22(21):5008-15 PMID: 6227337
  21. Chromatin structure of hsp 70 genes, activated by heat shock: selective removal of histones from the coding region and their absence from the 5' region.
    Cell. 1984 Feb;36(2):423-31 PMID: 6420075
  22. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  23. Differentiation-dependent chromatin alterations precede and accompany transcription of immunoglobulin light chain genes.
    J Biol Chem. 1984 Jul 10;259(13):8534-44 PMID: 6429143
  24. Differential salt fractionation of active and inactive genomic domains in chicken erythrocyte.
    J Biol Chem. 1984 Jul 10;259(13):8558-63 PMID: 6736042
  25. Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4275-9 PMID: 6379641
  26. Histone-H1-dependent chromatin superstructures and the suppression of gene activity.
    Cell. 1984 Aug;38(1):17-27 PMID: 6467367
  27. Histone H5 promotes the association of condensed chromatin fragments to give pseudo-higher-order structures.
    Eur J Biochem. 1985 Feb 15;147(1):143-51 PMID: 3971973
  28. Association of histone H1(0) with a gene repressed during liver development.
    Nature. 1985 Mar 14-20;314(6007):197-8 PMID: 2579343
  29. Primary organization of nucleosomes. Interaction of non-histone high mobility group proteins 14 and 17 with nucleosomes, as revealed by DNA-protein crosslinking and immunoaffinity isolation.
    J Mol Biol. 1985 Sep 20;185(2):329-39 PMID: 4057250
  30. Exchange of proteins during immunofractionation of chromatin.
    Exp Cell Res. 1986 Mar;163(1):95-102 PMID: 3510889
  31. General method for isolation of DNA sequences that interact with specific nuclear proteins in chromosomes: binding of the high mobility group protein HMG-T to a subset of the protamine gene family.
    Biochemistry. 1985 Dec 31;24(27):8021-8 PMID: 4092053
  32. Chromatin structure of the chicken lysozyme gene domain as determined by chromatin fractionation and micrococcal nuclease digestion.
    Biochemistry. 1986 Jan 28;25(2):495-502 PMID: 3955010
  33. High mobility group proteins 1 and 2 stimulate transcription in vitro by RNA polymerases II and III.
    J Biol Chem. 1986 May 25;261(15):6986-92 PMID: 3700424
  34. Isolation of oligonucleosomes from active chromatin using HMG17-specific monoclonal antibodies.
    Nucleic Acids Res. 1986 Apr 25;14(8):3363-76 PMID: 3703677
  35. Digestion of the chicken beta-globin gene chromatin with micrococcal nuclease reveals the presence of an altered nucleosomal array characterized by an atypical ladder of DNA fragments.
    EMBO J. 1986 Feb;5(2):293-300 PMID: 3011400
  36. Preferential in vitro binding of high mobility group proteins 14 and 17 to nucleosomes containing active and DNase I sensitive single-copy genes.
    Biochemistry. 1986 Jun 3;25(11):3447-54 PMID: 3730369
  37. Immunofractionation of DNA sequences associated with HMG-17 in chromatin.
    Exp Cell Res. 1986 Oct;166(2):486-96 PMID: 3743668
  38. The DNase I sensitive domain of the chicken lysozyme gene spans 24 kb.
    Nucleic Acids Res. 1986 Aug 11;14(15):6085-99 PMID: 3748804
  39. Immunofractionation of chromatin regions associated with histone H1o.
    Eur J Biochem. 1986 Oct 15;160(2):253-60 PMID: 2429835
  40. Exchange of H1 histone depends on aggregation of chromatin, not simply on ionic strength.
    J Biol Chem. 1986 Nov 25;261(33):15805-12 PMID: 3782092
  41. Selective solubilization of beta-globin oligonucleosomes at low ionic strength.
    Biochemistry. 1987 Jan 13;26(1):290-5 PMID: 3828304
  42. Selective radiolabelling and identification of a strong nucleosome binding site on the globular domain of histone H5.
    EMBO J. 1986 Dec 20;5(13):3531-7 PMID: 3104028
  43. Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
    Cell. 1987 Apr 24;49(2):203-10 PMID: 3568125
  44. Perturbation of chromatin structure in the region of the adult beta-globin gene in chicken erythrocyte chromatin.
    J Mol Biol. 1987 Jan 5;193(1):57-70 PMID: 3586025
  45. Deposition of newly synthesized histones: new histones H2A and H2B do not deposit in the same nucleosome with new histones H3 and H4.
    Biochemistry. 1987 Apr 21;26(8):2315-25 PMID: 3620448
  46. A bacteriophage RNA polymerase transcribes in vitro through a nucleosome core without displacing it.
    Cell. 1987 Aug 28;50(5):801-8 PMID: 3621345
  47. Chromatin from transcribed genes contains HMG17 only downstream from the starting point of transcription.
    EMBO J. 1987 Aug;6(8):2393-9 PMID: 3665881
  48. Anionic regions in nuclear proteins.
    J Cell Biol. 1987 Oct;105(4):1479-82 PMID: 3312230
  49. High mobility group proteins 1 and 2 stimulate binding of a specific transcription factor to the adenovirus major late promoter.
    Nucleic Acids Res. 1988 Feb 25;16(4):1471-86 PMID: 2831501
  50. Primary organization of nucleosome core particle of chromatin: sequence of histone arrangement along DNA.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4184-8 PMID: 279907
  51. Interaction of HMG 14 and 17 with actively transcribed genes.
    Cell. 1980 Jan;19(1):289-301 PMID: 6244103
  52. Primary organization of nucleosomes containing all five histones and DNA 175 and 165 base-pairs long.
    J Mol Biol. 1980 May 25;139(3):519-36 PMID: 7441743
  53. The interaction of high mobility proteins HMG14 and 17 with nucleosomes.
    Nucleic Acids Res. 1980 Sep 11;8(17):3757-78 PMID: 6449690
  54. The method of crosslinking histones to DNA partly depurinated at neutral pH.
    Anal Biochem. 1981 Jan 1;110(1):93-101 PMID: 7212274
  55. A new method for the isolation of replicative chromatin: selective deposition of histone on both new and old DNA.
    Cell. 1981 Jan;23(1):121-34 PMID: 7194149
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.4.717
Published
1991-02-25
Pages
717-25
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333702
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.4.717 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2