Home LiteratureArticle Details
PMID: 3399383 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Erythroid-specific gene chromatin has an altered association with linker histones.

Nucleic acids research ·Vol. 16 ·No. 13 ·1988-07-11 ·Pages 5915-26

Ridsdale JA, Rattner JB, Davie JR

Abstract

The chromatin of several genes was assayed for sensitivity to DNAase I and for solubility as polynucleosomes in 0.15 M NaCl. The degree of solubility of chromatin fragments as polynucleosomes in 0.15 M NaCl correlates well with the sensitivity to DNAase I for several genes. Chromatin of repressed, housekeeping and erythroid-specific genes can be distinguished as distinct groups by the degree to which they display these properties. NaCl precipitation of chromatin fragments stripped and then reconstituted with varying quantities of H1 and H5 (linker) histones indicate that the polynucleosomes of erythroid-specific genes have altered interaction with these histones. Linker histones interacted with bulk chromatin and in the chromatin of the repressed ovalbumin and vitellogenin genes to form salt precipitable structures. Chromatin of erythroid-specific genes (histone H5 and beta-globin) as well as that of the histone H2A.F gene was resistant to linker histone induced precipitation.

MeSH Terms
Animals Chickens Chromatin/drug effects Deoxyribonuclease I/metabolism Ethidium/pharmacology Histones/metabolism Microscopy, Electron Nucleosomes/analysis Osmolar Concentration Solubility
Chemicals
Chromatin Histones Nucleosomes Deoxyribonuclease I Ethidium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ridsdale J A
Department of Biochemistry, University of Manitoba, Winnipeg, Canada.
Rattner J B
Davie J R
References (34)
34 references, click to expand
  1. The ovalbumin gene: cloning of the natural gene.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3688-92 PMID: 358194
  2. Position-independent, high-level expression of the human beta-globin gene in transgenic mice.
    Cell. 1987 Dec 24;51(6):975-85 PMID: 3690667
  3. Reassociation of histone H1 with nucleosomes.
    J Biol Chem. 1979 Nov 25;254(22):11751-60 PMID: 500671
  4. Salt induced transitions of chromatin core particles studied by tyrosine fluorescence anisotropy.
    Nucleic Acids Res. 1980 Aug 25;8(16):3517-34 PMID: 7433098
  5. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  6. A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion.
    Cell. 1981 Nov;27(1 Pt 2):45-55 PMID: 6276024
  7. Participation of core histone "tails" in the stabilization of the chromatin solenoid.
    J Cell Biol. 1982 May;93(2):285-97 PMID: 7096439
  8. The chicken beta globin gene region. Delineation of transcription units and developmental regulation of interspersed DNA repeats.
    J Biol Chem. 1982 Sep 25;257(18):11015-23 PMID: 6286679
  9. Nucleosomal particles open as the histone core becomes hyperacetylated.
    Eur J Biochem. 1983 Feb 15;130(3):437-45 PMID: 6218991
  10. Characterization of the chicken vimentin gene: single copy gene producing multiple mRNAs.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):911-5 PMID: 6573660
  11. H2A.F: an extremely variant histone H2A sequence expressed in the chicken embryo.
    Proc Natl Acad Sci U S A. 1983 May;80(10):2819-23 PMID: 6574451
  12. Genomic organization of the genes coding for the six main histones of the chicken: complete sequence of the H5 gene.
    J Mol Biol. 1983 Nov 15;170(4):843-59 PMID: 6315952
  13. The role of stable complexes that repress and activate eucaryotic genes.
    Cell. 1984 Jun;37(2):359-65 PMID: 6722879
  14. Differential salt fractionation of active and inactive genomic domains in chicken erythrocyte.
    J Biol Chem. 1984 Jul 10;259(13):8558-63 PMID: 6736042
  15. Temporal order of chromatin structural changes associated with activation of the major chicken vitellogenin gene.
    Cell. 1983 May;33(1):65-76 PMID: 6088056
  16. Histone-H1-dependent chromatin superstructures and the suppression of gene activity.
    Cell. 1984 Aug;38(1):17-27 PMID: 6467367
  17. Torsional stress promotes the DNAase I sensitivity of active genes.
    Cell. 1984 Dec;39(3 Pt 2):469-78 PMID: 6096006
  18. N-Butyrate incubation of immature chicken erythrocytes preferentially enhances the solubility of beta A chromatin.
    Nucleic Acids Res. 1985 Mar 25;13(6):1977-95 PMID: 4000950
  19. Assembly and propagation of repressed and depressed chromosomal states.
    Cell. 1985 Oct;42(3):705-11 PMID: 2996773
  20. Localization of DNAase I-sensitive sequences to specific regions of interphase nuclei.
    Cell. 1985 Dec;43(2 Pt 1):471-82 PMID: 4075401
  21. The beta-globin domain in immature chicken erythrocytes: enhanced solubility is coincident with histone hyperacetylation.
    Nucleic Acids Res. 1986 Feb 25;14(4):1667-82 PMID: 3951993
  22. Digestion of the chicken beta-globin gene chromatin with micrococcal nuclease reveals the presence of an altered nucleosomal array characterized by an atypical ladder of DNA fragments.
    EMBO J. 1986 Feb;5(2):293-300 PMID: 3011400
  23. Hyperacetylation of core histones does not cause unfolding of nucleosomes. Neutron scatter data accords with disc shape of the nucleosome.
    J Biol Chem. 1986 Jul 5;261(19):8784-92 PMID: 3722174
  24. Structure of transcriptionally active chromatin.
    CRC Crit Rev Biochem. 1986;21(1):1-26 PMID: 3015490
  25. Binding of ethidium bromide causes dissociation of the nucleosome core particle.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8472-6 PMID: 3464964
  26. In situ detection of beta-galactosidase in lenses of transgenic mice with a gamma-crystallin/lacZ gene.
    Science. 1987 Jan 23;235(4787):456-8 PMID: 3099390
  27. Chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength and contain linker histones are highly enriched in beta-globin gene sequences.
    Nucleic Acids Res. 1987 Feb 11;15(3):1081-96 PMID: 3822820
  28. Pancreatic neoplasia induced by ras expression in acinar cells of transgenic mice.
    Cell. 1987 Mar 27;48(6):1023-34 PMID: 3470144
  29. Perturbation of chromatin structure in the region of the adult beta-globin gene in chicken erythrocyte chromatin.
    J Mol Biol. 1987 Jan 5;193(1):57-70 PMID: 3586025
  30. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  31. Factors affecting nucleosome disassembly by protamines in vitro. Histone hyperacetylation and chromatin structure, time dependence, and the size of the sperm nuclear proteins.
    J Biol Chem. 1987 Dec 15;262(35):17016-25 PMID: 3680288
  32. Regulation of histone and beta A-globin gene expression during differentiation of chicken erythroid cells.
    Mol Cell Biol. 1987 Oct;7(10):3663-72 PMID: 3119991
  33. Chicken erythrocyte beta-globin chromatin: enhanced solubility is a direct consequence of induced histone hyperacetylation.
    Nucleic Acids Res. 1987 Nov 25;15(22):9325-37 PMID: 3684594
  34. Higher order structure in metaphase chromosomes. I. The 250 A fiber.
    Chromosoma. 1978 Dec 6;69(3):363-72 PMID: 743904
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.13.5915
Published
1988-07-11
Pages
5915-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336837
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.13.5915 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2