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PMID: 743904 Published · ppublish English Journal Article

Higher order structure in metaphase chromosomes. I. The 250 A fiber.

Chromosoma ·Vol. 69 ·No. 3 ·1978-12-06 ·Pages 363-72

Rattner JB, Hamkalo BA

Abstract

Metaphase chromosomes released from cells in the presence of Joklik's suspension media by vortex-mixing with 0.5 mm glass beads have been analyzed by electron microscopy. In these preparations the chromosomes are composed of series of loops (200-300 A in diameter) which are, in turn, composed of closely-apposed arrays of nucleosomes. Negative-staining of these preparations has allowed the identification of several distinct patterns within the loop which appear to arise from variations in nucleosome packing. Analogous patterns are also observed in chromatin fragments generated by brief micrococcal nuclease digestion. From these data we have deduced certain features of nucleosome-nucleosome interactions in higher-ordered chromatin fibers.

MeSH Terms
Animals Cell Cycle Cell Line Chromosomes/ultrastructure Metaphase Mice Microscopy, Electron
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rattner J B
Hamkalo B A
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00332139
Published
1978-12-06
Pages
363-72
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332139 View journal details
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