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PMID: 1058483 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Chromatin architecture: investigation of a subunit of chromatin by dark field electron microscopy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2691-5

Langmore JP, Wooley JC

Abstract

Dark field scanning electron microscopy of unstained, unfixed samples of chromatin, histone-1-depleted chromatin, and nucleohistone has been used to identify an apparent subunit of chromatin, namely a disk-shaped structure we term the unit particle, which is probably about 135 A wide and 50 A thick in the hydrated state. The unit particles are found at rather uniform intervals along thin DNA-like fibers. Histone 1 depletion leads to a bimodal distribution of these spacings. Our observations suggest that the unit particle consists of a loop of nucleoprotein, perhaps around a histone core.

MeSH Terms
Animals Cattle Cell Nucleus/analysis,ultrastructure Chromatin/analysis,ultrastructure Chromatography, Gel Histones/analysis Microscopy, Electron Nucleoproteins/analysis Thymus Gland/analysis
Chemicals
Chromatin Histones Nucleoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Langmore J P
Wooley J C
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2691
Published
1975-07-00
Pages
2691-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432836
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2691 PMC Full Text View journal details
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