The chromosome fiber: evidence for an ordered superstructure of nucleosomes.

Chromosoma ·Vol. 62 ·No. 4 ·1977-07-18 ·Pages 301-17

Hozier J, Renz M, Nehls P

Abstract

Chromosome fibers isolated from lymphocyte nuclei and prepared for electron microscopy by techniques designed to preserve their native structure have a distinctly knobby appearance, suggesting that DNA and protein are not distributed evenly along the fiber axis. Individual knobs (superbeads) are arranged in tandem and have an average diameter of about 200 A. Mild nuclease digestion of isolated nuclei releases apparent monomer superbeads that are composed of nucleohistone particles with the properties of nucleosomes. The kinetics of digestion indicate that the superbead is a discrete structural unit containing, on the average, about eight nucleosomes.

MeSH Terms

Animals Cattle Chromosomes/ultrastructure DNA/analysis Histones Lymphocytes/ultrastructure Micrococcal Nuclease Microscopy, Electron Proteins/analysis

Chemicals

Histones Proteins DNA Micrococcal Nuclease

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hozier J

Renz M

Nehls P

References (36)

36 references, click to expand

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Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00327030

Published

1977-07-18

Pages

301-17

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00327030 View journal details

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The chromosome fiber: evidence for an ordered superstructure of nucleosomes.

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