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PMID: 279907 Published · ppublish English Journal Article

Primary organization of nucleosome core particle of chromatin: sequence of histone arrangement along DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 9 ·1978-09-00 ·Pages 4184-8

Mirzabekov AD, Shick VV, Belyavsky AV, Bavykin SG

Abstract

A high-resolution map for the arrangement of histones along DNA in the nucleosome core particle has been determined by a sequencing procedure based on crosslinking histones to the 5'-terminal DNA fragments produced by scission of one DNA strand at the point of crosslinking. The position of histones on DNA has been identified by measuring the length of crosslinked DNA fragments. The results demonstrate that each of the histones is arranged within several adjacent or dispersed DNA segments of a little less than 10 nucleotides in length. Histone-free intervals are located between these segments at the regular distances of about (10)n nucleotides from the 5' end of the DNA and are likely to face one side of the DNA helix. Histones appear to be arranged in a similar manner on both DNA strands and do not form "locks" around DNA. A linearized model of the core particle is proposed.

MeSH Terms
Binding Sites Cell Line Chromatin/ultrastructure DNA/metabolism Histones/metabolism Molecular Weight Protein Binding
Chemicals
Chromatin Histones DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mirzabekov A D
Shick V V
Belyavsky A V
Bavykin S G
References (25)
25 references, click to expand
  1. Mapping DNAase l-susceptible sites in nucleosomes labeled at the 5' ends.
    Cell. 1976 Oct;9(2):347-53 PMID: 975247
  2. Nucleosome structure II: structure of the SV40 minichromosome and electron microscopic evidence for reversible transitions of the nucleosome structure.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:301-12 PMID: 209940
  3. Crosslinked histone octamer as a model of the nucleosome core.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2780-4 PMID: 197520
  4. Chromatin.
    Nature. 1978 Jan 12;271(5641):115-22 PMID: 340956
  5. Structure of chromatin.
    Annu Rev Biochem. 1977;46:931-54 PMID: 332067
  6. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  7. The structure of nucleosomes: the arrangement of histones in the DNA grooves and along the DNA chain.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:149-55 PMID: 277336
  8. DNA folding in the nucleosome.
    J Mol Biol. 1977 Oct 15;116(1):49-71 PMID: 592385
  9. [Two simple methods for isolation of DNA from various sources using cetavlon].
    Biokhimiia. 1977 Oct;42(10):1783-90 PMID: 922066
  10. Structure of nucleosome core particles of chromatin.
    Nature. 1977 Sep 1;269(5623):29-36 PMID: 895884
  11. Protein arrangement in the DNA grooves in chromatin and nucleoprotamine in vitro and in vivo revealed by methylation.
    Eur J Biochem. 1977 May 16;75(2):379-89 PMID: 885136
  12. Nucleotide sequence of bacteriophage phi X174 DNA.
    Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
  13. Chromosomal proteins and chromatin structure.
    Annu Rev Biochem. 1975;44:725-74 PMID: 1094921
  14. Reactive properties of the amino groups of histones in calf thymus chromatin.
    J Mol Biol. 1974 Feb 5;82(4):537-45 PMID: 4817796
  15. Chromatin structure: a repeating unit of histones and DNA.
    Science. 1974 May 24;184(4139):868-71 PMID: 4825889
  16. The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.
    Cell. 1976 Jul;8(3):333-47 PMID: 986252
  17. Studies on chromatin. Free DNA in sheared chromatin.
    Eur J Biochem. 1976 JUL 1;66(2):211-23 PMID: 947750
  18. Heterogeneity of chromatin subunits in vitro and location of histone H1.
    Nucleic Acids Res. 1976 Feb;3(2):477-92 PMID: 1257057
  19. [Covalent binding of proteins with DNA in the chromatin structure].
    Dokl Akad Nauk SSSR. 1975 Apr 11;(5):1222-5 PMID: 1149603
  20. Cleavage of DNA in nuclei and chromatin with staphylococcal nuclease.
    Biochemistry. 1975 Jul;14(13):2921-5 PMID: 1148185
  21. A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease.
    Biochemistry. 1975 Jul;14(13):2915-20 PMID: 1148184
  22. Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt.
    Cell. 1975 Sep;6(1):85-110 PMID: 1164735
  23. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  24. A study of some factors that influence the iodination of ox insulin.
    Biochem J. 1967 Apr;103(1):120-8 PMID: 6033753
  25. Internal structure of the chromatin subunit.
    Nucleic Acids Res. 1974 Nov;1(11):1573-8 PMID: 10793712
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.9.4184
Published
1978-09-00
Pages
4184-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336076
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.9.4184 PMC Full Text View journal details
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