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PMID: 2740216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Influence of histone acetylation on the solubility, H1 content and DNase I sensitivity of newly assembled chromatin.

Nucleic acids research ·Vol. 17 ·No. 11 ·1989-06-12 ·Pages 4275-91

Perry CA, Annunziato AT

Abstract

In a previous report [Annunziato, A.T. and Seale, R.L. (1983) J. Biol. Chem. 258:12675] a novel intermediate in chromatin assembly was described (detected by labeling new DNA in the presence of the deacetylase inhibitor sodium butyrate), which retained approximately 50% of the heightened sensitivity of newly replicated chromatin to DNaseI. It is now reported that nucleosomes replicated in butyrate are considerably more soluble in the presence of magnesium, relative to chromatin replicated under control conditions, and that this heightened magnesium-solubility is reflected in a concomitant increase in the preferential solubility of nucleosomes containing newly synthesized core histones. This differential solubility was accompanied by a 5- to 6-fold depletion of histone H1, and was completely abolished by the selective removal of H1 from isolated nuclei. The removal of H1 also markedly reduced the preferential DNaseI sensitivity of chromatin replicated in butyrate. Further, when mononucleosomes of control and (acetylated) nascent chromatin were compared, no differences in DNaseI sensitivity were detected. These results provide evidence that the interactions between newly assembled nucleosomes and histone H1 are altered when histone deacetylation is inhibited during chromatin replication, and suggest a mechanism for the control of H1 deposition during nucleosome assembly in vivo.

MeSH Terms
Acetylation Butyrates/pharmacology Butyric Acid Chemical Fractionation Chromatin/biosynthesis,drug effects,metabolism DNA Replication/drug effects Deoxyribonuclease I HeLa Cells Histones/biosynthesis,metabolism Humans Nucleosomes/metabolism Solubility
Chemicals
Butyrates Chromatin Histones Nucleosomes Butyric Acid Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Perry C A
Department of Biology, Boston College, Chestnut Hill, MA 02167.
Annunziato A T
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.11.4275
Published
1989-06-12
Pages
4275-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317934
Subset
IM
Grants
NIGMS NIH HHS · GM35837 · United States
External Links
PubMed source DOI: 10.1093/nar/17.11.4275 PMC Full Text View journal details
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