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PMID: 11390659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Essential roles of Snf5p in Snf-Swi chromatin remodeling in vivo.

Molecular and cellular biology ·Vol. 21 ·No. 13 ·2001-07-00 ·Pages 4311-20

Geng F, Cao Y, Laurent BC

Abstract

Snf-Swi, the prototypical ATP-dependent nucleosome-remodeling complex, regulates transcription of a subset of yeast genes. With the exception of Snf2p, the ATPase subunit, the functions of the other components are unknown. We have investigated the role of the conserved Snf-Swi core subunit Snf5p through characterization of two conditional snf5 mutants. The mutants contain single amino acid alterations of invariant or conserved residues that abolish Snf-Swi-dependent transcription by distinct mechanisms. One mutation impairs Snf-Swi assembly and, consequently, its stable association with a target promoter. The other blocks a postrecruitment catalytic remodeling step. These findings suggest that Snf5p coordinates the assembly and nucleosome-remodeling activities of Snf-Swi.

MeSH Terms
Adenosine Triphosphate/metabolism Alleles Amino Acid Motifs Chromatin/genetics,metabolism Chromosomal Proteins, Non-Histone DNA/metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Genes, Reporter/genetics Glucose/pharmacology Glycoside Hydrolases/genetics,metabolism Humans Immunoblotting Macromolecular Substances Nucleosomes/genetics,metabolism Promoter Regions, Genetic Protein Binding/drug effects Protein Structure, Tertiary Recombinant Fusion Proteins/genetics,metabolism Regulatory Sequences, Nucleic Acid/genetics SMARCB1 Protein Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Temperature Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic/genetics beta-Fructofuranosidase
Chemicals
Chromatin Chromosomal Proteins, Non-Histone DNA-Binding Proteins Macromolecular Substances Nucleosomes Recombinant Fusion Proteins SMARCB1 Protein SMARCB1 protein, human SNF5 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Adenosine Triphosphate DNA Glycoside Hydrolases beta-Fructofuranosidase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Geng F
Department of Microbiology and Immunology and Morse Institute of Molecular Biology and Genetics, State University of New York, Brooklyn, New York 11203, USA.
Cao Y
Laurent B C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.13.4311-4320.2001
Published
2001-07-00
Pages
4311-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87091
Subset
IM
Grants
NIGMS NIH HHS · R01 GM056700 · United States
NIGMS NIH HHS · GM56700 · United States
External Links
PubMed source DOI: 10.1128/MCB.21.13.4311-4320.2001 PMC Full Text View journal details
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