Home LiteratureArticle Details
PMID: 10390216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Biochemical-genetic analysis and distribution of FAR-1, a class A beta-lactamase from Nocardia farcinica.

Antimicrobial agents and chemotherapy ·Vol. 43 ·No. 7 ·1999-07-00 ·Pages 1644-50

Laurent F, Poirel L, Naas T, Chaibi EB, Labia R, Boiron P, Nordmann P

Abstract

From genomic DNA of the clinical isolate Nocardia farcinica VIC, a 1. 6-kb Sau3AI fragment was cloned and expressed in Escherichia coli JM109. The recombinant strain expressed a beta-lactamase (pI, 4.6), FAR-1, which conferred high levels of resistance to amoxicillin, piperacillin, ticarcillin, and cephalothin. The hydrolysis constants (kcat, Km, Ki, and 50% inhibitory concentration) confirmed the MIC results and showed that FAR-1 activity is inhibited by clavulanic acid and at a low level by tazobactam and sulbactam. Moreover, FAR-1 beta-lactamase hydrolyzes aztreonam (at a low level) without significant activity against ceftazidime, cefotaxime and imipenem. FAR-1 mature protein of molecular mass ca 32 kDa, has less than 60% amino acid identity with any other class A beta-lactamases, being most closely related to PEN-A from Burkholderia cepacia (52%). A blaFAR-1-like gene was found in all studied N. farcinica strains, underlining the constitutive origin of this gene.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/pharmacology Base Sequence Cloning, Molecular Molecular Sequence Data Nocardia/enzymology beta-Lactamases/analysis,genetics,metabolism beta-Lactams
Chemicals
Anti-Bacterial Agents beta-Lactams beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Laurent F
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cédex, France.
Poirel L
Naas T
Chaibi E B
Labia R
Boiron P
Nordmann P
References (50)
50 references, click to expand
  1. The Aeromonas hydrophila cphA gene: molecular heterogeneity among class B metallo-beta-lactamases.
    J Bacteriol. 1991 Aug;173(15):4611-7 PMID: 1856163
  2. Comparison of the sequences of class A beta-lactamases and of the secondary structure elements of penicillin-recognizing proteins.
    Antimicrob Agents Chemother. 1991 Nov;35(11):2294-301 PMID: 1804001
  3. Review of nocardial infections in France 1987 to 1990.
    Eur J Clin Microbiol Infect Dis. 1992 Aug;11(8):709-14 PMID: 1425729
  4. Nocardia and nocardiosis.
    J Med Vet Mycol. 1992;30 Suppl 1:317-31 PMID: 1474457
  5. Genes for a beta-lactamase, a penicillin-binding protein and a transmembrane protein are clustered with the cephamycin biosynthetic genes in Nocardia lactamdurans.
    EMBO J. 1993 Feb;12(2):631-9 PMID: 8440253
  6. Partial characterization of Nocardia farcinica beta-lactamases.
    Antimicrob Agents Chemother. 1993 Sep;37(9):1850-5 PMID: 8239595
  7. The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences.
    Electrophoresis. 1993 Oct;14(10):1023-31 PMID: 8125050
  8. Sequence analysis of PER-1 extended-spectrum beta-lactamase from Pseudomonas aeruginosa and comparison with class A beta-lactamases.
    Antimicrob Agents Chemother. 1994 Jan;38(1):104-14 PMID: 8141562
  9. Structural and kinetic characterization of a beta-lactamase-inhibitor protein.
    Nature. 1994 Apr 14;368(6472):657-60 PMID: 8145854
  10. Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions.
    Electrophoresis. 1994 Mar-Apr;15(3-4):529-39 PMID: 8055880
  11. Transcription and expression analysis, using lacZ and phoA gene fusions, of Mycobacterium fortuitum beta-lactamase genes cloned from a natural isolate and a high-level beta-lactamase producer.
    Mol Microbiol. 1994 May;12(3):491-504 PMID: 8065266
  12. The medically important aerobic actinomycetes: epidemiology and microbiology.
    Clin Microbiol Rev. 1994 Jul;7(3):357-417 PMID: 7923055
  13. beta-Lactamase mutations far from the active site influence inhibitor binding.
    Biochim Biophys Acta. 1995 Feb 22;1247(1):121-5 PMID: 7873581
  14. Cloning, sequencing, and site-directed mutagenesis of beta-lactamase gene from Streptomyces fradiae Y59.
    Antimicrob Agents Chemother. 1995 Jan;39(1):260-3 PMID: 7535038
  15. Antigenic properties and immunoelectron microscopic localization of Mycobacterium fortuitum beta-lactamase.
    Antimicrob Agents Chemother. 1995 Mar;39(3):739-45 PMID: 7793883
  16. A functional classification scheme for beta-lactamases and its correlation with molecular structure.
    Antimicrob Agents Chemother. 1995 Jun;39(6):1211-33 PMID: 7574506
  17. Ribotyping: a tool for taxonomy and identification of the Nocardia asteroides complex species.
    J Clin Microbiol. 1996 May;34(5):1079-82 PMID: 8727879
  18. Selection and characterization of amino acid substitutions at residues 237-240 of TEM-1 beta-lactamase with altered substrate specificity for aztreonam and ceftazidime.
    J Biol Chem. 1996 Sep 13;271(37):22538-45 PMID: 8798421
  19. beta-lactamase gene promoters of 71 clinical strains of Klebsiella oxytoca.
    Antimicrob Agents Chemother. 1996 Feb;40(2):460-3 PMID: 8834898
  20. Implication of Ile-69 and Thr-182 residues in kinetic characteristics of IRT-3 (TEM-32) beta-lactamase.
    Antimicrob Agents Chemother. 1996 Oct;40(10):2434-6 PMID: 8891161
  21. Occurrence of plasmids in pathogenic strains of Nocardia.
    J Med Microbiol. 1996 Nov;45(5):344-8 PMID: 8918949
  22. Evolution and dissemination of beta-lactamases accelerated by generations of beta-lactam antibiotics.
    Clin Infect Dis. 1997 Jan;24 Suppl 1:S19-45 PMID: 8994778
  23. Molecular diversity and evolution of blaTEM genes encoding beta-lactamases resistant to clavulanic acid in clinical E. coli.
    J Mol Evol. 1997 Jan;44(1):57-65 PMID: 9010136
  24. Properties of IRT-14 (TEM-45), a newly characterized mutant of TEM-type beta-lactamases.
    Antimicrob Agents Chemother. 1997 Feb;41(2):374-8 PMID: 9021194
  25. Nomenclature of TEM beta-lactamases.
    J Antimicrob Chemother. 1997 Jan;39(1):1-3 PMID: 9044020
  26. Comparison of agar dilution, broth microdilution, disk diffusion, E-test, and BACTEC radiometric methods for antimicrobial susceptibility testing of clinical isolates of the Nocardia asteroides complex.
    J Clin Microbiol. 1997 Apr;35(4):847-52 PMID: 9157140
  27. Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra.
    Antimicrob Agents Chemother. 1997 May;41(5):1182-5 PMID: 9145897
  28. Non-inducible, mainly cell-associated beta-lactamase from Nocardia asteroides strain 108.
    J Antimicrob Chemother. 1997 Jul;40(1):5-11 PMID: 9249198
  29. The bla gene of the cephamycin cluster of Streptomyces clavuligerus encodes a class A beta-lactamase of low enzymatic activity.
    J Bacteriol. 1997 Oct;179(19):6035-40 PMID: 9324249
  30. Characterization of the penA and penR genes of Burkholderia cepacia 249 which encode the chromosomal class A penicillinase and its LysR-type transcriptional regulator.
    Antimicrob Agents Chemother. 1997 Nov;41(11):2399-405 PMID: 9371340
  31. Nature of monocyclic beta-lactam antibiotic Nocardicin A to beta-lactamases.
    Microbiol Immunol. 1988;32(2):119-30 PMID: 3287105
  32. Inhibitors and inactivators of beta-lactamase from Mycobacterium fortuitum.
    J Chemother. 1989 Oct;1(5):293-7 PMID: 2585031
  33. Nucleotide sequence of the gene encoding the active-site serine beta-lactamase from Actinomadura R39.
    FEMS Microbiol Lett. 1989 Dec;53(3):241-6 PMID: 2515104
  34. Lipoproteins in bacteria.
    J Bioenerg Biomembr. 1990 Jun;22(3):451-71 PMID: 2202727
  35. Molecular analysis of beta-lactamases from four species of Streptomyces: comparison of amino acid sequences with those of other beta-lactamases.
    J Gen Microbiol. 1990 Mar;136(3):589-98 PMID: 2391494
  36. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  37. Letter: Nocardicin A and B, novel monocyclic beta-lactam antibiotics from a Nocardia species.
    J Am Chem Soc. 1976 May 12;98(10):3023-5 PMID: 1262632
  38. The structure of beta-lactamases.
    Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321-31 PMID: 6109327
  39. Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium.
    Nucleic Acids Res. 1981 Jun 11;9(11):2577-88 PMID: 6269055
  40. Use of trimethoprim-sulfamethoxazole for treatment of infections due to Nocardia.
    Rev Infect Dis. 1982 Mar-Apr;4(2):315-25 PMID: 6981158
  41. Trimethoprim-sulfamethoxazole therapy for Nocardia infections.
    Arch Intern Med. 1983 Apr;143(4):711-8 PMID: 6340623
  42. A cephalosporin active in vivo against Nocardia: efficacy of cefotaxime in murine model of acute pulmonary nocardiosis.
    J Hyg (Lond). 1983 Dec;91(3):421-7 PMID: 6319484
  43. Nucleotide sequence of the Staphylococcus aureus PC1 beta-lactamase gene.
    Nucleic Acids Res. 1986 Jul 25;14(14):5940 PMID: 3488540
  44. Beta-lactam resistance in Nocardia brasiliensis is mediated by beta-lactamase and reversed in the presence of clavulanic acid.
    J Infect Dis. 1987 Dec;156(6):959-66 PMID: 3500241
  45. beta-lactamase I from Bacillus cereus. Structure and site-directed mutagenesis.
    Biochem J. 1987 Dec 15;248(3):657-62 PMID: 3124817
  46. Characterization of a beta-lactamase produced in Mycobacterium fortuitum D316.
    Biochem J. 1990 Nov 1;271(3):729-34 PMID: 2123098
  47. Therapy of pulmonary nocardiosis in immunocompromised mice.
    Antimicrob Agents Chemother. 1990 Sep;34(9):1766-8 PMID: 2285288
  48. Substitution of lysine at position 104 or 240 of TEM-1pTZ18R beta-lactamase enhances the effect of serine-164 substitution on hydrolysis or affinity for cephalosporins and the monobactam aztreonam.
    Biochemistry. 1991 Apr 2;30(13):3179-88 PMID: 1901218
  49. Molecular evolution of class A beta-lactamases: phylogeny and patterns of sequence conservation.
    Mol Microbiol. 1990 Nov;4(11):1957-65 PMID: 2082152
  50. Acquired resistance of Nocardia brasiliensis to clavulanic acid related to a change in beta-lactamase following therapy with amoxicillin-clavulanic acid.
    Antimicrob Agents Chemother. 1991 Mar;35(3):524-8 PMID: 2039203
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.43.7.1644
Published
1999-07-00
Pages
1644-50
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC89337
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.43.7.1644 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2