Home LiteratureArticle Details
PMID: 1804001 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of the sequences of class A beta-lactamases and of the secondary structure elements of penicillin-recognizing proteins.

Antimicrobial agents and chemotherapy ·Vol. 35 ·No. 11 ·1991-11-00 ·Pages 2294-301

Joris B, Ledent P, Dideberg O, Fonzé E, Lamotte-Brasseur J, Kelly JA, Ghuysen JM, Frère JM

Abstract

The sequences of class A beta-lactamases were compared. Four main groups of enzymes were distinguished: those from the gram-negative organisms and bacilli and two distinct groups of Streptomyces spp. The Staphylococcus aureus PC1 enzyme, although somewhat closer to the enzyme from the Bacillus group, did not belong to any of the groups of beta-lactamases. The similarities between the secondary structure elements of these enzymes and those of the class C beta-lactamases and of the Streptomyces sp. strain R61 DD-peptidase were also analyzed and tentatively extended to the class D beta-lactamases. A unified nomenclature of secondary structure elements is proposed for all the penicillin-recognizing enzymes.

MeSH Terms
Amino Acid Sequence Bacterial Proteins Carrier Proteins/analysis Gram-Negative Bacteria/enzymology Hexosyltransferases Molecular Sequence Data Muramoylpentapeptide Carboxypeptidase/analysis Penicillin-Binding Proteins Penicillins/analysis Peptidyl Transferases Protein Conformation Streptomyces/enzymology X-Ray Diffraction beta-Lactamases/analysis,chemistry
Chemicals
Bacterial Proteins Carrier Proteins Penicillin-Binding Proteins Penicillins Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase beta-Lactamases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Joris B
Laboratoire d'Enzymologie, Université de Liège, Belgium.
Ledent P
Dideberg O
Fonzé E
Lamotte-Brasseur J
Kelly J A
Ghuysen J M
Frère J M
References (46)
46 references, click to expand
  1. A general method applicable to the search for similarities in the amino acid sequence of two proteins.
    J Mol Biol. 1970 Mar;48(3):443-53 PMID: 5420325
  2. Tn552, a novel transposable element from Staphylococcus aureus.
    Mol Microbiol. 1990 Jun;4(6):961-75 PMID: 2170815
  3. Mechanism of acyl transfer by the class A serine beta-lactamase of Streptomyces albus G.
    Biochem J. 1991 Oct 1;279 ( Pt 1):213-21 PMID: 1930139
  4. A standard numbering scheme for the class A beta-lactamases.
    Biochem J. 1991 May 15;276 ( Pt 1):269-70 PMID: 2039479
  5. Refined crystal structure of beta-lactamase from Staphylococcus aureus PC1 at 2.0 A resolution.
    J Mol Biol. 1991 Feb 20;217(4):701-19 PMID: 2005620
  6. Site-directed mutagenesis of beta-lactamase I. Single and double mutants of Glu-166 and Lys-73.
    Biochem J. 1990 Dec 15;272(3):613-9 PMID: 1980064
  7. Engineering a novel beta-lactamase by a single point mutation.
    Protein Eng. 1990 Oct;4(1):79-86 PMID: 2127105
  8. Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase.
    Biochem J. 1990 Dec 15;272(3):627-31 PMID: 2125210
  9. OHIO-1 beta-lactamase is part of the SHV-1 family.
    Antimicrob Agents Chemother. 1990 Aug;34(8):1570-6 PMID: 2121093
  10. Molecular evolution of class A beta-lactamases: phylogeny and patterns of sequence conservation.
    Mol Microbiol. 1990 Nov;4(11):1957-65 PMID: 2082152
  11. Nucleotide sequence of the Serratia marcescens SR50 chromosomal ampC beta-lactamase gene.
    FEMS Microbiol Lett. 1990 Aug;58(3):295-9 PMID: 2227364
  12. Chromosomal beta-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics.
    Antimicrob Agents Chemother. 1989 Jan;33(1):63-70 PMID: 2653216
  13. Sequence and comparative analysis of three Enterobacter cloacae ampC beta-lactamase genes and their products.
    Biochem J. 1988 Mar 15;250(3):753-60 PMID: 3260487
  14. Single amino acid substitution between SHV-1 beta-lactamase and cefotaxime-hydrolyzing SHV-2 enzyme.
    FEBS Lett. 1988 Apr 11;231(1):217-20 PMID: 3129309
  15. Sequence of the Citrobacter freundii OS60 chromosomal ampC beta-lactamase gene.
    Eur J Biochem. 1986 May 2;156(3):441-5 PMID: 3486121
  16. Crystallographic mapping of beta-lactams bound to a D-alanyl-D-alanine peptidase target enzyme.
    J Mol Biol. 1989 Sep 20;209(2):281-95 PMID: 2585485
  17. Tertiary structural similarity between a class A beta-lactamase and a penicillin-sensitive D-alanyl carboxypeptidase-transpeptidase.
    Nature. 1986 Mar 27-Apr 2;320(6060):378-80 PMID: 3485771
  18. Precise insertion of antibiotic resistance determinants into Tn21-like transposons: nucleotide sequence of the OXA-1 beta-lactamase gene.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7378-82 PMID: 2823258
  19. Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins.
    Nucleic Acids Res. 1986 Aug 26;14(16):6745-63 PMID: 3092189
  20. Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene.
    Eur J Biochem. 1987 Feb 2;162(3):509-18 PMID: 3830154
  21. Penicillin-binding proteins of gram-negative bacteria.
    Rev Infect Dis. 1988 Jul-Aug;10(4):699-711 PMID: 3055170
  22. The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 DD-peptidase family.
    Biochem J. 1988 Mar 1;250(2):313-24 PMID: 3128280
  23. Cloning and sequencing of the blaZ gene encoding beta-lactamase III, a lipoprotein of Bacillus cereus 569/H.
    J Bacteriol. 1987 Feb;169(2):579-86 PMID: 3027036
  24. Nucleotide sequence of the gene encoding the Streptomyces albus G beta-lactamase precursor.
    Eur J Biochem. 1987 Jul 15;166(2):345-50 PMID: 3038538
  25. Cloning and sequencing of the beta-lactamase I gene of Bacillus cereus 5/B and its expression in Bacillus subtilis.
    J Bacteriol. 1985 Aug;163(2):487-92 PMID: 2991192
  26. Sequence of PSE-2 beta-lactamase.
    Antimicrob Agents Chemother. 1988 Jan;32(1):134-6 PMID: 3126705
  27. Nucleotide sequence of the gene encoding the active-site serine beta-lactamase from Actinomadura R39.
    FEMS Microbiol Lett. 1989 Dec;53(3):241-6 PMID: 2515104
  28. The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170.
    Arch Microbiol. 1989;151(2):91-4 PMID: 2655551
  29. The phototrophic bacterium Rhodopseudomonas capsulata sp108 encodes an indigenous class A beta-lactamase.
    Biochem J. 1989 Jun 15;260(3):803-12 PMID: 2788410
  30. Complete amino acid sequence of p453-plasmid-mediated PIT-2 beta-lactamase (SHV-1).
    Biochem J. 1988 Apr 1;251(1):73-9 PMID: 3260490
  31. Close evolutionary relationship between the chromosomally encoded beta-lactamase gene of Klebsiella pneumoniae and the TEM beta-lactamase gene mediated by R plasmids.
    FEBS Lett. 1986 Oct 20;207(1):69-74 PMID: 3533626
  32. The crystal structure of the beta-lactamase of Streptomyces albus G at 0.3 nm resolution.
    Biochem J. 1987 Aug 1;245(3):911-3 PMID: 3499147
  33. Sequence of the OXA2 beta-lactamase: comparison with other penicillin-reactive enzymes.
    FEBS Lett. 1985 Oct 21;191(1):39-44 PMID: 3876949
  34. Direct sequencing of the amplified structural gene and promoter for the extended-broad-spectrum beta-lactamase TEM-9 (RHH-1) of Klebsiella pneumoniae.
    Plasmid. 1990 Jan;23(1):27-34 PMID: 2161546
  35. Expression in Escherichia coli of the carboxy terminal domain of the BLAR sensory-transducer protein of Bacillus licheniformis as a water-soluble Mr 26,000 penicillin-binding protein.
    FEMS Microbiol Lett. 1990 Jun 15;58(1):107-13 PMID: 2204571
  36. Identification of BlaR, the signal transducer for beta-lactamase production in Bacillus licheniformis, as a penicillin-binding protein with strong homology to the OXA-2 beta-lactamase (class D) of Salmonella typhimurium.
    J Bacteriol. 1990 Feb;172(2):1137-41 PMID: 2404938
  37. Substitution of aspartic acid-217 of Citrobacter freundii cephalosporinase and properties of the mutant enzymes.
    FEBS Lett. 1990 May 21;264(2):211-4 PMID: 1972682
  38. Sequence analysis and evolutionary perspectives of ROB-1 beta-lactamase.
    Antimicrob Agents Chemother. 1990 Jul;34(7):1354-9 PMID: 2201253
  39. Molecular analysis of beta-lactamases from four species of Streptomyces: comparison of amino acid sequences with those of other beta-lactamases.
    J Gen Microbiol. 1990 Mar;136(3):589-98 PMID: 2391494
  40. Beta-lactamase of Bacillus licheniformis 749/C at 2 A resolution.
    Proteins. 1990;7(2):156-71 PMID: 2326252
  41. Refined crystal structure of beta-lactamase from Citrobacter freundii indicates a mechanism for beta-lactam hydrolysis.
    Nature. 1990 Jan 18;343(6255):284-8 PMID: 2300174
  42. Nucleotide sequence of the PSE-4 carbenicillinase gene and correlations with the Staphylococcus aureus PC1 beta-lactamase crystal structure.
    J Biol Chem. 1990 Jan 15;265(2):1225-30 PMID: 2295609
  43. The structure of beta-lactamases.
    Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321-31 PMID: 6109327
  44. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  45. Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries.
    Nucleic Acids Res. 1982 Jan 11;10(1):247-63 PMID: 6801626
  46. ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4897-901 PMID: 6795623
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.35.11.2294
Published
1991-11-00
Pages
2294-301
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC245374
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.35.11.2294 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2