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PMID: 9145897 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra.

Antimicrobial agents and chemotherapy ·Vol. 41 ·No. 5 ·1997-05-00 ·Pages 1182-5

Hackbarth CJ, Unsal I, Chambers HF

Abstract

A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.

MeSH Terms

Amino Acid Sequence Cefoxitin/pharmacology Cephamycins/pharmacology Cloning, Molecular Cosmids Isoelectric Focusing Molecular Sequence Data Mycobacterium tuberculosis/enzymology,genetics Sequence Analysis beta-Lactamases/classification,drug effects,genetics

Chemicals

Cephamycins Cefoxitin beta-Lactamases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hackbarth C J

Department of Medicine, University of California, San Francisco 94143, USA. corinne@itsa.ucsf.edu

Unsal I

Chambers H F

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.41.5.1182

corinne@itsa.ucsf.edu

Published

1997-05-00

Pages

1182-5

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC163878

Subset

Grants

NIAID NIH HHS · R01AI33702 · United States

Databases

GENBANK

U67924

External Links

PubMed source DOI: 10.1128/AAC.41.5.1182 PMC Full Text View journal details

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Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra.

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