Home LiteratureArticle Details
PMID: 8891161 Published · ppublish English Journal Article

Implication of Ile-69 and Thr-182 residues in kinetic characteristics of IRT-3 (TEM-32) beta-lactamase.

Antimicrobial agents and chemotherapy ·Vol. 40 ·No. 10 ·1996-10-00 ·Pages 2434-6

Farzaneh S, Chaibi EB, Peduzzi J, Barthelemy M, Labia R, Blazquez J, Baquero F

Abstract

The substitution of a methionine for an isoleucine at position 69 (Met69Ile), which causes inhibitor resistance to TEM-type beta-lactamases (IRT-3 and IRT-I69), altered the positions of the Asn-170 and Glu-166 side chains as well as the position of the catalytic water molecule. A novel hydrogen bond between the hydroxyl of Thr-182 and the carbonyl of Glu-64 was expected to be responsible for the increase in the catalytic activity of the IST-T182 and IRT-3 enzymes compared with those of TEM-1 and IRT-169, respectively.

MeSH Terms
Catalysis Cephalosporins/metabolism Isoleucine/chemistry,metabolism Kinetics Penicillins/metabolism Threonine/chemistry,metabolism beta-Lactamases/chemistry,metabolism
Chemicals
Cephalosporins Penicillins Isoleucine Threonine beta-lactamase IRT-3 beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Farzaneh S
URA 401 Centre National de la Recherche Scientifique,-MNHN, Quimper, France.
Chaibi E B
Peduzzi J
Barthelemy M
Labia R
Blazquez J
Baquero F
References (20)
20 references, click to expand
  1. Molecular characterization of nine different types of mutants among 107 inhibitor-resistant TEM beta-lactamases from clinical isolates of Escherichia coli.
    Antimicrob Agents Chemother. 1995 Feb;39(2):427-30 PMID: 7726509
  2. beta-Lactamase mutations far from the active site influence inhibitor binding.
    Biochim Biophys Acta. 1995 Feb 22;1247(1):121-5 PMID: 7873581
  3. Extended-spectrum and inhibitor-resistant TEM-type beta-lactamases: mutations, specificity, and three-dimensional structure.
    Antimicrob Agents Chemother. 1995 Dec;39(12):2593-601 PMID: 8592985
  4. Computerized microacidimetric determination of beta lactamase Michaelis-Menten constants.
    FEBS Lett. 1973 Jun 15;33(1):42-4 PMID: 4198636
  5. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41 PMID: 358200
  6. An efficient method for generating proteins with altered enzymatic properties: application to beta-lactamase.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9094-8 PMID: 2687873
  7. A standard numbering scheme for the class A beta-lactamases.
    Biochem J. 1991 May 15;276 ( Pt 1):269-70 PMID: 2039479
  8. TRC-1: emergence of a clavulanic acid-resistant TEM beta-lactamase in a clinical strain.
    FEMS Microbiol Lett. 1992 Mar 1;70(2):113-7 PMID: 1316862
  9. OHIO-1 beta-lactamase resistant to mechanism-based inactivators.
    FEMS Microbiol Lett. 1992 Apr 1;71(1):79-82 PMID: 1320587
  10. Site-directed mutagenesis at the active site of Escherichia coli TEM-1 beta-lactamase. Suicide inhibitor-resistant mutants reveal the role of arginine 244 and methionine 69 in catalysis.
    J Biol Chem. 1992 Oct 15;267(29):20600-6 PMID: 1400382
  11. Clinical isolates of Escherichia coli producing TRI beta-lactamases: novel TEM-enzymes conferring resistance to beta-lactamase inhibitors.
    J Antimicrob Chemother. 1992 Oct;30(4):449-62 PMID: 1490918
  12. Selection of variants of the TEM-1 beta-lactamase, encoded by a plasmid of clinical origin, with increased resistance to beta-lactamase inhibitors.
    J Antimicrob Chemother. 1993 May;31(5):655-64 PMID: 8392995
  13. Crystal structure of Escherichia coli TEM1 beta-lactamase at 1.8 A resolution.
    Proteins. 1993 Aug;16(4):364-83 PMID: 8356032
  14. Chromosomally encoded cephalosporin-hydrolyzing beta-lactamase of Proteus vulgaris RO104 belongs to Ambler's class A.
    Biochim Biophys Acta. 1994 Jul 20;1207(1):31-9 PMID: 8043607
  15. Characterization and amino acid sequence of IRT-4, a novel TEM-type enzyme with a decreased susceptibility to beta-lactamase inhibitors.
    FEMS Microbiol Lett. 1994 Jul 1;120(1-2):111-7 PMID: 8056282
  16. Nucleotide sequences of the genes coding for the TEM-like beta-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2).
    FEMS Microbiol Lett. 1994 Jul 1;120(1-2):75-80 PMID: 8056297
  17. Emergence of clinical isolates of Escherichia coli producing TEM-1 derivatives or an OXA-1 beta-lactamase conferring resistance to beta-lactamase inhibitors.
    Antimicrob Agents Chemother. 1994 May;38(5):1085-9 PMID: 8067742
  18. Clinical isolates of Escherichia coli producing multiple TEM mutants resistant to beta-lactamase inhibitors.
    J Antimicrob Chemother. 1994 Jun;33(6):1117-26 PMID: 7928805
  19. Complementary roles of mutations at positions 69 and 242 in a class A beta-lactamase.
    Biochim Biophys Acta. 1995 Feb 22;1247(1):113-20 PMID: 7873579
  20. Incidence and mechanisms of resistance to the combination of amoxicillin and clavulanic acid in Escherichia coli.
    Antimicrob Agents Chemother. 1995 Nov;39(11):2478-83 PMID: 8585729
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.40.10.2434
Published
1996-10-00
Pages
2434-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC163551
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.40.10.2434 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2