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PMID: 9891087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SH3P7 is a cytoskeleton adapter protein and is coupled to signal transduction from lymphocyte antigen receptors.

Molecular and cellular biology ·Vol. 19 ·No. 2 ·1999-02-00 ·Pages 1539-46

Larbolette O, Wollscheid B, Schweikert J, Nielsen PJ, Wienands J

Abstract

Lymphocytes respond to antigen receptor engagement with tyrosine phosphorylation of many cellular proteins, some of which have been identified and functionally characterized. Here we describe SH3P7, a novel substrate protein for Src and Syk family kinases. SH3P7 migrates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a 55-kDa protein that is preferentially expressed in brain, thymus, and spleen. It contains multiple amino acid sequence motifs, including two consensus tyrosine phosphorylation sites of the YXXP type and one SH3 domain. A region of sequence similarity, which we named SCAD, was found in SH3P7 and three actin-binding proteins. The SCAD region may represent a new type of protein-protein interaction domain that mediates binding to actin. Consistent with this possibility, SH3P7 colocalizes with actin filaments of the cytoskeleton. Altogether, our data implicate SH3P7 as an adapter protein which links antigen receptor signaling to components of the cytoskeleton.

MeSH Terms
3T3 Cells Actins/metabolism Amino Acid Sequence Animals B-Lymphocytes/immunology,metabolism Base Sequence Binding Sites/genetics Cell Line Cytoskeletal Proteins/genetics,immunology,metabolism DNA Primers/genetics Lymphocyte Activation Mice Microfilament Proteins/genetics,immunology,metabolism Molecular Sequence Data Phosphorylation Protein Binding Receptors, Antigen, B-Cell/metabolism Sequence Homology, Amino Acid Signal Transduction Tyrosine/chemistry src Homology Domains src-Family Kinases/metabolism
Chemicals
Actins Cytoskeletal Proteins DNA Primers Dbnl protein, mouse Microfilament Proteins Receptors, Antigen, B-Cell Tyrosine src-Family Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Larbolette O
Abteilung für Molekulare Immunologie, Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, and Max-Planck-Institut für Immunbiologie, D-79108 Freiburg, Germany.
Wollscheid B
Schweikert J
Nielsen P J
Wienands J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.19.2.1539
Published
1999-02-00
Pages
1539-46
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC116082
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.19.2.1539 PMC Full Text View journal details
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