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PMID: 7682714 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 8 ·1993-04-15 ·Pages 3631-5

Yamanashi Y, Okada M, Semba T, Yamori T, Umemori H, Tsunasawa S, Toyoshima K, Kitamura D, Watanabe T, Yamamoto T

Abstract

Crosslinking of membrane-bound immunoglobulins, which are B-cell antigen receptors, causes proliferation and differentiation of B cells or inhibition of their growth. The receptor-mediated signaling involves tyrosine phosphorylation of cellular proteins and rapid activation of Src-like kinases. The amino acid sequences of five proteolytic peptides of p75, a major substrate of protein-tyrosine(s) in the signaling, showed that p75 is the human HS1 gene product. The HS1 gene is expressed specifically in hematopoietic cells and encodes p75HS1, which carries both helix-turn-helix and Src homology 3 motifs. p75HS1 showed rapid tyrosine phosphorylation and association with a Src-like kinase, Lyn, after crosslinking of membrane-bound IgM. Thus, p75HS1 may be an important substrate of Lyn and possibly other protein-tyrosine kinases upon B-cell antigen receptor-mediated signaling.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Antibodies Antibodies, Monoclonal B-Lymphocytes/immunology,physiology Blood Proteins/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Humans Immunoblotting Leukocyte Common Antigens/isolation & purification Molecular Sequence Data Molecular Weight Peptide Fragments/isolation & purification Phosphoproteins/isolation & purification,metabolism Phosphotyrosine Protein-Tyrosine Kinases/metabolism Receptors, Antigen, T-Cell/physiology Signal Transduction Tumor Cells, Cultured Tyrosine/analogs & derivatives,analysis
Chemicals
Adaptor Proteins, Signal Transducing Antibodies Antibodies, Monoclonal Blood Proteins HCLS1 protein, human Peptide Fragments Phosphoproteins Receptors, Antigen, T-Cell Phosphotyrosine Tyrosine Protein-Tyrosine Kinases Leukocyte Common Antigens
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Yamanashi Y
Department of Oncology, University of Tokyo, Japan.
Okada M
Semba T
Yamori T
Umemori H
Tsunasawa S
Toyoshima K
Kitamura D
Watanabe T
Yamamoto T
References (29)
29 references, click to expand
  1. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  2. The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck.
    Mol Cell Biol. 1987 Jan;7(1):237-43 PMID: 3561390
  3. Isolation and characterization of a novel human gene expressed specifically in the cells of hematopoietic lineage.
    Nucleic Acids Res. 1989 Nov 25;17(22):9367-79 PMID: 2587259
  4. A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis.
    Cell. 1990 Jan 26;60(2):307-17 PMID: 2404612
  5. Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc2+.
    Cell. 1990 Jan 26;60(2):319-28 PMID: 2297790
  6. Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins.
    Nature. 1990 Mar 1;344(6261):36-42 PMID: 2137557
  7. Association of B cell antigen receptor with protein tyrosine kinase Lyn.
    Science. 1991 Jan 11;251(4990):192-4 PMID: 1702903
  8. Oncogenes and signal transduction.
    Cell. 1991 Jan 25;64(2):281-302 PMID: 1846320
  9. Tyrosine phosphorylation of phospholipase C induced by membrane immunoglobulin in B lymphocytes.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2745-9 PMID: 2011584
  10. IgM antigen receptor complex contains phosphoprotein products of B29 and mb-1 genes.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3982-6 PMID: 2023945
  11. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.
    Science. 1991 May 3;252(5006):668-74 PMID: 1708916
  12. Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src.
    Nature. 1991 May 2;351(6321):69-72 PMID: 1709258
  13. Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7410-4 PMID: 1714601
  14. Molecular cloning of a porcine gene syk that encodes a 72-kDa protein-tyrosine kinase showing high susceptibility to proteolysis.
    J Biol Chem. 1991 Aug 25;266(24):15790-6 PMID: 1874735
  15. A protein-tyrosine phosphatase with sequence similarity to the SH2 domain of the protein-tyrosine kinases.
    Nature. 1991 Aug 22;352(6337):736-9 PMID: 1652101
  16. The B-cell antigen receptor complex.
    Immunol Today. 1991 Jun;12(6):196-201 PMID: 1878135
  17. Signalling in B cells.
    Immunol Today. 1991 Jun;12(6):201-5 PMID: 1652257
  18. Identification and characterization of a novel cytoskeleton-associated pp60src substrate.
    Mol Cell Biol. 1991 Oct;11(10):5113-24 PMID: 1922035
  19. Insulin-like growth factor I rapidly induces tyrosine phosphorylation of a Mr 150,000 and a Mr 160,000 protein in highly metastatic mouse colon carcinoma 26 NL-17 cells.
    Cancer Res. 1991 Nov 1;51(21):5859-65 PMID: 1718586
  20. Engagement of the high-affinity IgE receptor activates src protein-related tyrosine kinases.
    Nature. 1992 Jan 2;355(6355):78-80 PMID: 1370575
  21. Activation of Src-like protein-tyrosine kinase Lyn and its association with phosphatidylinositol 3-kinase upon B-cell antigen receptor-mediated signaling.
    Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1118-22 PMID: 1371009
  22. Membrane Ig cross-linking regulates phosphatidylinositol 3-kinase in B lymphocytes.
    J Immunol. 1992 Apr 1;148(7):2012-22 PMID: 1372019
  23. Differential responses of p56lyn and p53lyn, products of alternatively spliced lyn mRNA, on stimulation of B-cell antigen receptor.
    Cell Regul. 1991 Dec;2(12):979-87 PMID: 1801928
  24. Association of the 72-kDa protein-tyrosine kinase PTK72 with the B cell antigen receptor.
    J Biol Chem. 1992 Apr 25;267(12):8613-9 PMID: 1569106
  25. Association between B-lymphocyte membrane immunoglobulin and multiple members of the Src family of protein tyrosine kinases.
    Mol Cell Biol. 1992 May;12(5):2315-21 PMID: 1569953
  26. Tyrosine phosphorylation of phospholipase C-gamma 2 upon cross-linking of membrane Ig on murine B lymphocytes.
    J Immunol. 1992 May 15;148(10):3021-7 PMID: 1578127
  27. Predominant expression and activation-induced tyrosine phosphorylation of phospholipase C-gamma 2 in B lymphocytes.
    Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5660-4 PMID: 1376928
  28. Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2936-40 PMID: 6930676
  29. Selective expression of a protein-tyrosine kinase, p56lyn, in hematopoietic cells and association with production of human T-cell lymphotropic virus type I.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6538-42 PMID: 2505253
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.8.3631
Published
1993-04-15
Pages
3631-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46355
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.8.3631 PMC Full Text View journal details
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