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PMID: 1371009 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activation of Src-like protein-tyrosine kinase Lyn and its association with phosphatidylinositol 3-kinase upon B-cell antigen receptor-mediated signaling.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 3 ·1992-02-01 ·Pages 1118-22

Yamanashi Y, Fukui Y, Wongsasant B, Kinoshita Y, Ichimori Y, Toyoshima K, Yamamoto T

Abstract

Crosslinking of membrane-bound immunoglobulins, which are B-cell antigen receptors, causes proliferation and differentiation of B cells or the inhibition of their growth. The receptor-mediated signaling involves tyrosine phosphorylation of cellular proteins. The Src-like protein-tyrosine kinase Lyn is expressed preferentially in B cells and is an intracytoplasmic constituent of the B-cell antigen receptor complex. Crosslinking of membrane-bound immunoglobulin M with antibody induced rapid increases in the kinase activities of Lyn and Lyn-associated phosphatidylinositol 3-kinase. Crosslinking of B-cell antigen receptor also induced association of Lyn with an 85-kDa noncatalytic subunit of phosphatidylinositol 3-kinase. Thus, Lyn is functionally associated with membrane-bound immunoglobulin M and seems likely to participate in B-cell antigen receptor-mediated signaling.

MeSH Terms
B-Lymphocytes/physiology Enzyme Activation Humans Immunoglobulin M/physiology In Vitro Techniques Phosphatidylinositol 3-Kinases Phosphoproteins/metabolism Phosphotransferases/metabolism Phosphotyrosine Protein-Tyrosine Kinases/physiology Receptor Aggregation Receptors, Antigen, B-Cell/metabolism,physiology Signal Transduction Tumor Cells, Cultured Tyrosine/analogs & derivatives,metabolism Up-Regulation src-Family Kinases
Chemicals
Immunoglobulin M Phosphoproteins Receptors, Antigen, B-Cell Phosphotyrosine Tyrosine Phosphotransferases Phosphatidylinositol 3-Kinases Protein-Tyrosine Kinases lyn protein-tyrosine kinase src-Family Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yamanashi Y
Department of Oncology, University of Tokyo, Japan.
Fukui Y
Wongsasant B
Kinoshita Y
Ichimori Y
Toyoshima K
Yamamoto T
References (52)
52 references, click to expand
  1. Involvement of a guanine-nucleotide-binding component in membrane IgM-stimulated phosphoinositide breakdown.
    J Immunol. 1987 Dec 1;139(11):3604-13 PMID: 2824610
  2. Alternatively spliced murine lyn mRNAs encode distinct proteins.
    Mol Cell Biol. 1991 Jul;11(7):3399-406 PMID: 1710766
  3. Molecular analysis of the dissociation between IL-2 production and proliferation in a response of a T cell clone to the antigen presented by B cells.
    J Immunol. 1988 Nov 15;141(10):3278-84 PMID: 3263419
  4. Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain.
    Cell. 1980 Jun;20(2):303-12 PMID: 6771019
  5. The regulation of growth and differentiation of a murine B cell lymphoma. II. The inhibition of WEHI 231 by anti-immunoglobulin antibodies.
    J Immunol. 1981 Jun;126(6):2466-9 PMID: 6785356
  6. mRNA for surface immunoglobulin gamma chains encodes a highly conserved transmembrane sequence and a 28-residue intracellular domain.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):2008-12 PMID: 6804950
  7. Stimulation of protein tyrosine phosphorylation by the B-lymphocyte antigen receptor.
    Nature. 1990 Jun 28;345(6278):810-3 PMID: 1694265
  8. Binding of transforming protein, P47gag-crk, to a broad range of phosphotyrosine-containing proteins.
    Science. 1990 Jun 22;248(4962):1537-9 PMID: 1694307
  9. Enhancement of T-cell responsiveness by the lymphocyte-specific tyrosine protein kinase p56lck.
    Nature. 1991 Mar 7;350(6313):62-6 PMID: 1706070
  10. Tyrosine phosphorylation of components of the B-cell antigen receptors following receptor crosslinking.
    Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3436-40 PMID: 1707541
  11. Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7410-4 PMID: 1714601
  12. Oncogenes and signal transduction.
    Cell. 1991 Jan 25;64(2):281-302 PMID: 1846320
  13. Requirement of phosphatidylinositol-3 kinase modification for its association with p60src.
    Mol Cell Biol. 1991 Apr;11(4):1972-9 PMID: 1848666
  14. Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases.
    Cell. 1991 Apr 5;65(1):83-90 PMID: 1849461
  15. Two types of phosphatidylinositol 3-kinase from bovine thymus. Monomer and heterodimer form.
    J Biol Chem. 1991 May 5;266(13):8108-14 PMID: 1850737
  16. Regulation of T cell receptor signaling by a src family protein-tyrosine kinase (p59fyn).
    Cell. 1991 Apr 19;65(2):281-91 PMID: 2015626
  17. IgM antigen receptor complex contains phosphoprotein products of B29 and mb-1 genes.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3982-6 PMID: 2023945
  18. Co-capping of ras proteins with surface immunoglobulins in B lymphocytes.
    Nature. 1990 Sep 27;347(6291):396-400 PMID: 2120590
  19. B lymphocyte antigen receptors (mIg) are non-covalently associated with a disulfide linked, inducibly phosphorylated glycoprotein complex.
    EMBO J. 1990 Feb;9(2):441-8 PMID: 2154371
  20. Signal transduction by receptors with tyrosine kinase activity.
    Cell. 1990 Apr 20;61(2):203-12 PMID: 2158859
  21. Molecular components of the B cell antigen receptor complex of class IgD differ partly from those of IgM.
    EMBO J. 1990 Feb;9(2):449-55 PMID: 2303036
  22. Molecular components of the B-cell antigen receptor complex of the IgM class.
    Nature. 1990 Feb 22;343(6260):760-2 PMID: 2304550
  23. B lymphocyte receptors and polyphosphoinositide degradation.
    Cell. 1985 Jul;41(3):999-1006 PMID: 2408764
  24. The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex.
    Proc Natl Acad Sci U S A. 1989 May;86(9):3277-81 PMID: 2470098
  25. Selective expression of a protein-tyrosine kinase, p56lyn, in hematopoietic cells and association with production of human T-cell lymphotropic virus type I.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6538-42 PMID: 2505253
  26. Involvement of a specific guanine nucleotide binding protein in receptor immunoglobulin stimulated inositol phospholipid hydrolysis.
    Biochim Biophys Acta. 1989 Oct 9;1013(3):273-8 PMID: 2508752
  27. The colony stimulating factor-1 receptor associates with and activates phosphatidylinositol-3 kinase.
    Nature. 1989 Dec 7;342(6250):699-702 PMID: 2556641
  28. Signal transduction through the CD4 receptor involves the activation of the internal membrane tyrosine-protein kinase p56lck.
    Nature. 1989 Mar 16;338(6212):257-9 PMID: 2784195
  29. Receptor signalling and crosstalk in B lymphocytes.
    Immunol Rev. 1987 Oct;99:19-38 PMID: 2824341
  30. G protein coupling of antigen receptor-stimulated polyphosphoinositide hydrolysis in B cells.
    J Immunol. 1988 May 1;140(9):3135-9 PMID: 2834452
  31. Membrane IgM, IgD, and IgG act as signal transmission molecules in a series of B lymphomas.
    J Immunol. 1986 Oct 1;137(7):2162-7 PMID: 3020122
  32. The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck.
    Mol Cell Biol. 1987 Jan;7(1):237-43 PMID: 3561390
  33. Nucleotide sequences of gene segments encoding membrane domains of immunoglobulin gamma chains.
    Proc Natl Acad Sci U S A. 1982 Apr;79(8):2623-7 PMID: 6283537
  34. Regulation of murine B cells through surface immunoglobulin. I. Monoclonal anti-delta antibody that induces allotype-specific proliferation.
    J Exp Med. 1980 Nov 1;152(5):1135-46 PMID: 6776225
  35. Modeling of T cell contact-dependent B cell activation. IL-4 and antigen receptor ligation primes quiescent B cells to mobilize calcium in response to Ia cross-linking.
    J Immunol. 1991 Apr 1;146(7):2075-82 PMID: 1848577
  36. cDNA cloning of a novel 85 kd protein that has SH2 domains and regulates binding of PI3-kinase to the PDGF beta-receptor.
    Cell. 1991 Apr 5;65(1):75-82 PMID: 1849460
  37. Tyrosine phosphorylation of phospholipase C induced by membrane immunoglobulin in B lymphocytes.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2745-9 PMID: 2011584
  38. Hematopoietic cells express two forms of lyn kinase differing by 21 amino acids in the amino terminus.
    Mol Cell Biol. 1991 May;11(5):2391-8 PMID: 2017160
  39. Interaction of the IL-2 receptor with the src-family kinase p56lck: identification of novel intermolecular association.
    Science. 1991 Jun 14;252(5012):1523-8 PMID: 2047859
  40. Activation of phosphatidylinositol 3-kinase by insulin.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1411-5 PMID: 2154747
  41. Association of the fyn protein-tyrosine kinase with the T-cell antigen receptor.
    Proc Natl Acad Sci U S A. 1990 Jun;87(11):4358-62 PMID: 2190221
  42. Protein tyrosine phosphorylation is induced in murine B lymphocytes in response to stimulation with anti-immunoglobulin.
    EMBO J. 1990 Jul;9(7):2125-31 PMID: 2357961
  43. Isolation of antibodies for phosphotyrosine by immunization with a v-abl oncogene-encoded protein.
    Mol Cell Biol. 1985 Dec;5(12):3640-3 PMID: 2427929
  44. Antiimmunoglobulin inhibition of Burkitt's lymphoma cell proliferation and concurrent reduction of c-myc and mu heavy chain gene expression.
    Cancer Res. 1989 Jun 15;49(12):3235-41 PMID: 2497974
  45. Phosphatidylinositol kinase type I activity associates with various oncogene products.
    Oncogene Res. 1989;4(4):283-92 PMID: 2549489
  46. Increased phosphatidylinositol metabolism is an important but not an obligatory early event in B lymphocyte activation.
    J Immunol. 1985 Jun;134(6):4087-94 PMID: 2580904
  47. Structure of genes for membrane and secreted murine IgD heavy chains.
    Nature. 1982 Apr 1;296(5856):410-5 PMID: 6801528
  48. Frequency of B lymphocytes responsive to anti-immunoglobulin.
    J Exp Med. 1982 May 1;155(5):1523-36 PMID: 6802927
  49. Regulation of B cell antigen receptor signal transduction and phosphorylation by CD45.
    Science. 1991 Jun 28;252(5014):1839-42 PMID: 1648262
  50. Association of B cell antigen receptor with protein tyrosine kinase Lyn.
    Science. 1991 Jan 11;251(4990):192-4 PMID: 1702903
  51. Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase.
    Cell. 1991 Apr 5;65(1):91-104 PMID: 1707345
  52. Protein-tyrosine kinases.
    Annu Rev Biochem. 1985;54:897-930 PMID: 2992362
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.3.1118
Published
1992-02-01
Pages
1118-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48397
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.3.1118 PMC Full Text View journal details
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