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PMID: 9362070 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 8 ·No. 11 ·1997-11-00 ·Pages 2291-306

Wesp A, Hicke L, Palecek J, Lombardi R, Aust T, Munn AL, Riezman H

Abstract

end4-1 was isolated as a temperature-sensitive endocytosis mutant. We cloned and sequenced END4 and found that it is identical to SLA2/MOP2. This gene is required for growth at high temperature, viability in the absence of Abp1p, polarization of the cortical actin cytoskeleton, and endocytosis. We used a mutational analysis of END4 to correlate in vivo functions with regions of End4p and we found that two regions of End4p participate in endocytosis but that the talin-like domain of End4p is dispensable. The N-terminal domain of End4p is required for growth at high temperature, endocytosis, and actin organization. A central coiled-coil domain of End4p is necessary for formation of a soluble sedimentable complex. Furthermore, this domain has an endocytic function that is redundant with the function(s) of ABP1 and SRV2. The endocytic function of Abp1p depends on its SH3 domain. In addition we have isolated a recessive negative allele of SRV2 that is defective for endocytosis. Combined biochemical, functional, and genetic analysis lead us to propose that End4p may mediate endocytosis through interaction with other actin-associated proteins, perhaps Rvs167p, a protein essential for endocytosis.

MeSH Terms
Actins/metabolism Adaptor Proteins, Signal Transducing Amino Acid Sequence Carrier Proteins/chemistry,genetics,metabolism Cell Cycle Proteins Cloning, Molecular Cytoskeletal Proteins DNA-Binding Proteins/genetics,physiology Drosophila Proteins Endocytosis/physiology Fungal Proteins/chemistry,genetics,metabolism Microfilament Proteins Molecular Sequence Data Molecular Weight Peptides/chemistry Phenotype Protein Structure, Tertiary Recombinant Fusion Proteins Saccharomyces cerevisiae/cytology,genetics,growth & development Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Sequence Deletion Temperature Transcription Factors
Chemicals
ABF1 protein, S cerevisiae Actins Adaptor Proteins, Signal Transducing Capt protein, Drosophila Carrier Proteins Cell Cycle Proteins Cytoskeletal Proteins DNA-Binding Proteins Drosophila Proteins Fungal Proteins Microfilament Proteins Peptides Recombinant Fusion Proteins SLA2 protein, S cerevisiae SRV2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Transcription Factors cbp1 protein, S pombe
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wesp A
Biozentrum, University of Basel, Switzerland.
Hicke L
Palecek J
Lombardi R
Aust T
Munn A L
Riezman H
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.8.11.2291
Published
1997-11-00
Pages
2291-306
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25709
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.8.11.2291 PMC Full Text View journal details
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