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PMID: 8590801 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 6 ·No. 12 ·1995-12-00 ·Pages 1721-42

Munn AL, Stevenson BJ, Geli MI, Riezman H

Abstract

Four mutants defective in endocytosis were isolated by screening a collection of temperature-sensitive yeast mutants. Three mutations define new END genes: end5-1, end6-1, and end7-1. The fourth mutation is in END4, a gene identified previously. The end5-1, end6-1, and end7-1 mutations do not affect vacuolar protein localization, indicating that the defect in each mutant is specific for internalization at the plasma membrane. Interestingly, localization of actin patches on the plasma membrane is affected in each of the mutants. end5-1, end6-1, and end7-1 are allelic to VRP1, RVS161, and ACT1, respectively. VRP1 and RVS161 are required for correct actin localization and ACT1 encodes actin. To our surprise, the end6-1 mutation fails to complement the act1-1 mutation. Disruption of the RVS167 gene, which is homologous to END6/RVS161 and which is also required for correct actin localization, also blocks endocytosis. The end7-1 mutant allele has a glycine 48 to aspartic acid substitution in the DNase I-binding loop of actin. We propose that Vrp1p, Rvs161p, and Rvs167p are components of a cytoskeletal structure that contains actin and fimbrin and that is required for formation of endocytic vesicles at the plasma membrane.

MeSH Terms
Actins/biosynthesis,chemistry,genetics Alleles Amino Acid Sequence Base Sequence Biological Transport Chromosome Mapping Chromosomes, Fungal Crosses, Genetic Cytoskeletal Proteins DNA Primers Endocytosis/genetics,physiology Fungal Proteins/biosynthesis,genetics Genes, Fungal Genetic Complementation Test Genetic Linkage Genotype Kinetics Mating Factor Microfilament Proteins Models, Molecular Molecular Sequence Data Mutagenesis Peptides/metabolism Pheromones/metabolism Plasmids Polymerase Chain Reaction Protein Structure, Secondary Saccharomyces cerevisiae/genetics,growth & development,physiology Saccharomyces cerevisiae Proteins Temperature
Chemicals
Actins Cytoskeletal Proteins DNA Primers Fungal Proteins Microfilament Proteins Peptides Pheromones RVS161 protein, S cerevisiae Saccharomyces cerevisiae Proteins VRP1 protein, S cerevisiae Mating Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Munn A L
Department of Biochemistry, Biozentrum of the University of Basel, Switzerland.
Stevenson B J
Geli M I
Riezman H
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.6.12.1721
Published
1995-12-00
Pages
1721-42
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC301328
Subset
IM
Databases
GENBANK
X87806
External Links
PubMed source DOI: 10.1091/mbc.6.12.1721 PMC Full Text View journal details
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