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PMID: 2190989 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast.

The Journal of cell biology ·Vol. 110 ·No. 6 ·1990-06-00 ·Pages 1911-22

Singer B, Riezman H

Abstract

alpha-Factor, one of the mating pheromones of Saccharomyces cerevisiae, binds specifically to a receptor on the plasma membrane of a cells, is internalized and delivered to the vacuole, where it is degraded. At 15 degrees C the rate of pheromone uptake is only slightly affected while delivery to the vacuole is markedly slowed down. A transport intermediate carrying alpha-factor to the vacuole can be reversibly trapped by treatment with the metabolic inhibitors, NaN3 and NaF. This intermediate(s) is distinct from the vacuole and the plasma membrane as judged by differential and density gradient centrifugation analysis. We present evidence that the alpha-factor is protected from protease digestion by a detergent-sensitive structure, suggesting that the pheromone resides within a vesicular compartment. We propose that this intermediate(s) represents an endocytic or prevacuolar compartment(s) involved in vesicular traffic from the plasma membrane to the vacuole.

MeSH Terms
Animals Biological Transport/physiology Cell Membrane/metabolism,physiology,ultrastructure Centrifugation, Density Gradient Endocytosis Mating Factor Organelles/metabolism,physiology,ultrastructure Peptides/analysis,metabolism,pharmacokinetics Saccharomyces cerevisiae/metabolism,physiology Sodium Fluoride/pharmacology Sodium Nitrite/pharmacology Temperature Vacuoles/metabolism,physiology,ultrastructure
Chemicals
Peptides Mating Factor Sodium Fluoride Sodium Nitrite
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Singer B
Biocenter, University of Basel, Switzerland.
Riezman H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.110.6.1911
Published
1990-06-00
Pages
1911-22
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116120
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.110.6.1911 PMC Full Text View journal details
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