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PMID: 3887136 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Alpha-factor structural gene mutations in Saccharomyces cerevisiae: effects on alpha-factor production and mating.

Molecular and cellular biology ·Vol. 5 ·No. 4 ·1985-04-00 ·Pages 787-96

Kurjan J

Abstract

The role of alpha-factor structural genes MF alpha 1 and MF alpha 2 in alpha-factor production and mating has been investigated by the construction of mf alpha 1 and mf alpha 2 mutations that totally eliminate gene function. An mf alpha 1 mutant in which the entire coding region is deleted shows a considerable decrease in alpha-factor production and a 75% decrease in mating. Mutations in mf alpha 2 have little or no effect on alpha-factor production or mating. The mf alpha 1 mf alpha 2 double mutants are completely defective in mating and alpha-factor production. These results indicate that at least one alpha-factor structural gene product is required for mating in MAT alpha cells, that MF alpha 1 is responsible for the majority of alpha-factor production, and that MF alpha 1 and MF alpha 2 are the only active alpha-factor genes.

MeSH Terms
Base Sequence Cloning, Molecular Fungal Proteins/genetics Genes Genes, Fungal Mating Factor Mutation Peptides/genetics Reproduction Saccharomyces cerevisiae/genetics
Chemicals
Fungal Proteins Peptides Mating Factor
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kurjan J
References (33)
33 references, click to expand
  1. Comparison of dose-response curves for alpha factor-induced cell division arrest, agglutination, and projection formation of yeast cells. Implication for the mechanism of alpha factor action.
    J Biol Chem. 1983 Nov 25;258(22):13849-56 PMID: 6358212
  2. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.
    Nature. 1979 Mar 29;278(5703):423-7 PMID: 221818
  3. Two adrenal opioid polypeptides: proposed intermediates in the processing of proenkephalin.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1962-6 PMID: 6940201
  4. Control of yeast cell type by the mating type locus. II. Genetic interactions between MAT alpha and unlinked alpha-specific STE genes.
    J Mol Biol. 1981 Dec 5;153(2):323-35 PMID: 7040682
  5. Isolation and characterization of four related peptides exhibiting alpha factor activity from Saccharomyces cerevisiae.
    Eur J Biochem. 1976 May 17;65(1):257-62 PMID: 776628
  6. The structure of transposable yeast mating type loci.
    Cell. 1980 Mar;19(3):753-64 PMID: 6244896
  7. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. I. Isolation and phenotypic characterization of nonmating mutants.
    Genetics. 1974 Feb;76(2):255-71 PMID: 4595643
  8. Recovery of S. cerevisiae a cells from G1 arrest by alpha factor pheromone requires endopeptidase action.
    Cell. 1979 Nov;18(3):623-35 PMID: 391400
  9. Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.
    J Cell Biol. 1980 Jun;85(3):811-22 PMID: 6993497
  10. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  11. Saccharomyces cerevisiae: a diffusible sex factor.
    Science. 1970 Jun 19;168(3938):1472-3 PMID: 5445939
  12. Inactivation and chemical alteration of mating factor alpha by cells and spheroplasts of yeast.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1304-8 PMID: 16592511
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  15. Mutants of Saccharomyces cerevisiae resistant to the alpha mating-type factor.
    Genetics. 1976 Apr;82(4):639-44 PMID: 773748
  16. Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor.
    Cell. 1984 Jul;37(3):1075-89 PMID: 6430565
  17. Degradation of mating factor by alpha-mating type cells of Saccharomyces cerevisiae.
    J Biochem. 1977 Dec;82(6):1689-93 PMID: 340453
  18. Extracellular suppression allows mating by pheromone-deficient sterile mutants of Saccharomyces cerevisiae.
    J Bacteriol. 1983 Aug;155(2):903-6 PMID: 6348031
  19. Transfer of proteins across membranes.
    Annu Rev Biochem. 1981;50:317-48 PMID: 7023361
  20. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.
    Mol Cell Biol. 1982 Jan;2(1):11-20 PMID: 7050665
  21. Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity.
    Cell. 1980 Jul;20(3):701-9 PMID: 6998562
  22. Synchronization of haploid yeast cell cycles, a prelude to conjugation.
    Exp Cell Res. 1973 Jan;76(1):111-7 PMID: 4566309
  23. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor.
    Exp Cell Res. 1973 Jan;76(1):99-110 PMID: 4566314
  24. A functional prepro-alpha-factor gene in Saccharomyces yeasts can contain three, four, or five repeats of the mature pheromone sequence.
    Mol Cell Biol. 1983 Aug;3(8):1440-50 PMID: 6353204
  25. Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
    Cell. 1982 Oct;30(3):933-43 PMID: 6754095
  26. Glycosylation and processing of prepro-alpha-factor through the yeast secretory pathway.
    Cell. 1984 Feb;36(2):309-18 PMID: 6420074
  27. Yeast alpha factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase.
    Cell. 1983 Mar;32(3):839-52 PMID: 6339075
  28. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  29. Saccharomyces cerevisiae contains two discrete genes coding for the alpha-factor pheromone.
    Nucleic Acids Res. 1983 Jun 25;11(12):4049-63 PMID: 6306574
  30. Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA.
    Nature. 1982 Feb 25;295(5851):663-6 PMID: 7057924
  31. The role of S. cerevisiae cell division cycle genes in nuclear fusion.
    Genetics. 1982 Feb;100(2):175-84 PMID: 7049831
  32. Pancreatic preproglucagon cDNA contains two glucagon-related coding sequences arranged in tandem.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):345-9 PMID: 7043459
  33. Processing mechanisms in the biosynthesis of proteins.
    Ann N Y Acad Sci. 1980;343:1-16 PMID: 6994549
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.4.787-796.1985
Published
1985-04-00
Pages
787-96
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366783
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.5.4.787-796.1985 PMC Full Text View journal details
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