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PMID: 4075403 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reconstitution of an endocytic fusion event in a cell-free system.

Cell ·Vol. 43 ·No. 3 Pt 2 ·1985-12-00 ·Pages 643-52

Davey J, Hurtley SM, Warren G

Abstract

Using a cell-free system we have obtained fusion of vesicles from the endocytic pathway. The fusion is rapid, efficient, and requires ATP. Only vesicles derived from certain positions along the endocytic pathway are capable of fusing. Lysosomes and vesicles derived from the plasma membrane do not fuse.

MeSH Terms

Adenosine Triphosphate/physiology Animals Cell Line Cell Membrane/physiology Cell-Free System Cricetinae Endocytosis Influenza A virus Kidney Lysosomes/physiology Membrane Fusion Mesocricetus Semliki forest virus Sialic Acids/analysis

Chemicals

Sialic Acids Adenosine Triphosphate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Davey J

Hurtley S M

Warren G

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(85)90236-3

Published

1985-12-00

Pages

643-52

Language

English

Region

United States

NLM ID

0413066

Subset

External Links

PubMed source DOI: 10.1016/0092-8674(85)90236-3 View journal details

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Reconstitution of an endocytic fusion event in a cell-free system.

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