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PMID: 1315784 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein.

The Journal of cell biology ·Vol. 117 ·No. 5 ·1992-06-00 ·Pages 1067-76

Amatruda JF, Cooper JA

Abstract

Capping protein binds the barbed ends of actin filaments and nucleates actin filament assembly in vitro. We purified capping protein from Saccharomyces cervisiae. One of the two subunits is the product of the CAP2 gene, which we previously identified as the gene encoding the beta subunit of capping protein based on its sequence similarity to capping protein beta subunits in chicken and Dictyostelium (Amatruda, J. F., J. F. Cannon, K. Tatchell, C. Hug, and J. A. Cooper. 1990. Nature (Lond.) 344:352-354). Yeast capping protein has activity in critical concentration and low-shear viscometry assays consistent with barbed-end capping activity. Like chicken capping protein, yeast capping protein is inhibited by PIP2. By immunofluorescence microscopy yeast capping protein colocalizes with cortical actin spots at the site of bud emergence and at the tips of growing buds and shmoos. In contrast, capping protein does not colocalize with actin cables or with actin rings at the site of cytokinesis.

MeSH Terms
Actin Depolymerizing Factors Actins/analysis,isolation & purification,metabolism Destrin Microfilament Proteins/analysis,isolation & purification,metabolism Microscopy, Fluorescence Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols/metabolism Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/chemistry,growth & development
Chemicals
Actin Depolymerizing Factors Actins Destrin Microfilament Proteins Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols Recombinant Fusion Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Amatruda J F
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Cooper J A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.117.5.1067
Published
1992-06-00
Pages
1067-76
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289475
Subset
IM
Grants
NIGMS NIH HHS · GM 38542 · United States
Databases
GENBANK
M31165, M31166, M74319, M74320, M74321, M74322, M74323, M74324, X61398, X62630
External Links
PubMed source DOI: 10.1083/jcb.117.5.1067 PMC Full Text View journal details
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