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PMID: 2404021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells.

The Journal of cell biology ·Vol. 110 ·No. 1 ·1990-01-00 ·Pages 105-14

Haarer BK, Lillie SH, Adams AE, Magdolen V, Bandlow W, Brown SS

Abstract

We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Müller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, lose the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions.

MeSH Terms
Actins/isolation & purification,metabolism Amino Acid Sequence Chromatography, Affinity Chromosome Deletion Cloning, Molecular Contractile Proteins/isolation & purification Escherichia coli/genetics Fluorescent Antibody Technique Genes, Fungal Kinetics Macromolecular Substances Microfilament Proteins/genetics,isolation & purification,metabolism Mutation Profilins Restriction Mapping Saccharomyces cerevisiae/genetics,growth & development,ultrastructure
Chemicals
Actins Contractile Proteins Macromolecular Substances Microfilament Proteins Profilins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Haarer B K
Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor 48109.
Lillie S H
Adams A E
Magdolen V
Bandlow W
Brown S S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.110.1.105
Published
1990-01-00
Pages
105-14
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115986
Subset
IM
Grants
NIGMS NIH HHS · GM21253 · United States
NIGMS NIH HHS · GM34967 · United States
NICHD NIH HHS · HD07274 · United States
External Links
PubMed source DOI: 10.1083/jcb.110.1.105 PMC Full Text View journal details
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