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PMID: 7729411 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi.

The EMBO journal ·Vol. 14 ·No. 7 ·1995-04-03 ·Pages 1329-39

Schimmöller F, Singer-Krüger B, Schröder S, Krüger U, Barlowe C, Riezman H

Abstract

Emp24p is a type I transmembrane protein that is involved in secretory protein transport from the endoplasmic reticulum (ER) to the Golgi complex. A yeast mutant that lacks Emp24p (emp24 delta) is viable, but periplasmic invertase and the glycosylphosphatidyl-inositol-anchored plasma membrane protein Gas1p are delivered to the Golgi apparatus with reduced kinetics, whereas transport of alpha-factor, acid phosphatase and two vacuolar proteins is unaffected. Oligomerization and protease digestion studies of invertase suggest that the selective transport phenotype observed in the emp24 delta mutant is not due to a defect in protein folding or oligomerization. Consistent with a role in ER to Golgi transport, Emp24p is a component of COPII-coated, ER-derived transport vesicles that are isolated from a reconstituted in vitro budding reaction. We propose that Emp24p is involved in the sorting and/or concentration of a subset of secretory proteins into ER-derived transport vesicles.

Related Genes
EMP24
MeSH Terms
Amino Acid Sequence Carrier Proteins/analysis,chemistry,metabolism Cell Membrane/metabolism Cloning, Molecular Coated Pits, Cell-Membrane/metabolism Endoplasmic Reticulum/metabolism Genes, Fungal Glycoside Hydrolases/chemistry,metabolism Glycosylphosphatidylinositols/metabolism Golgi Apparatus/metabolism Kinetics Mating Factor Membrane Proteins/analysis,chemistry,metabolism Molecular Sequence Data Peptides/analysis,metabolism Pheromones/metabolism Protein Processing, Post-Translational Recombinant Proteins/analysis,chemistry,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Subcellular Fractions/metabolism Vesicular Transport Proteins beta-Fructofuranosidase
Chemicals
Carrier Proteins EMP24 protein, S cerevisiae Glycosylphosphatidylinositols Membrane Proteins Peptides Pheromones Recombinant Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins Mating Factor Glycoside Hydrolases beta-Fructofuranosidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schimmöller F
Biozentrum, University of Basel, Switzerland.
Singer-Krüger B
Schröder S
Krüger U
Barlowe C
Riezman H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-04-03
Pages
1329-39
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398218
Subset
IM
Databases
GENBANK
S40896, U00059, X67317
External Links
PubMed source PMC Full Text View journal details
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